LOCUS       sequence-164945-        4527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RELB , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_RELB_WT
FEATURES             Location/Qualifiers
     source          1..4527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1927..2026)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2043..2062)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2044..2062)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2067..2083)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2067..2083)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2514..3305
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3398..3986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3887..3906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4140..4157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4316..4343)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4435..4521)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gcttcggtct gggccagcct ctgggccgtc cgtccccact ggccgggcca
      241 tgccgagtcg ccgcgtcgcc agaccgccgg ctgcgccgga gctgggggcc ttagggtccc
      301 ccgacctctc ctcactctcg ctcgccgttt ccaggagcac agatgaattg gagatcatcg
      361 acgagtacat caaggagaac ggcttcggcc tggacggggg acagccgggc ccgggcgagg
      421 ggctgccacg cctggtgtct cgcggggctg cgtccctgag cacggtcacc ctgggccctg
      481 tggcgccccc agccacgccg ccgccttggg gctgccccct gggccgacta gtgtccccag
      541 cgccgggccc gggcccgcag ccgcacctgg tcatcacgga gcagcccaag cagcgcggca
      601 tgcgcttccg ctacgagtgc gagggccgct cggccggcag catccttggg gagagcagca
      661 ccgaggccag caagacgctg cccgccatcg agctccggga ttgtggaggg ctgcgggagg
      721 tggaggtgac tgcctgcctg gtgtggaagg actggcctca ccgagtccac ccccacagcc
      781 tcgtggggaa agactgcacc gacggcatct gcagggtgcg gctccggcct cacgtcagcc
      841 cccggcacag ttttaacaac ctgggcatcc agtgtgtgag gaagaaggag attgaggctg
      901 ccattgagcg gaagattcaa ctgggcattg acccctacaa cgctgggtcc ctgaagaacc
      961 atcaggaagt agacatgaat gtggtgagga tctgcttcca ggcctcatat cgggaccagc
     1021 agggacagat gcgccggatg gatcctgtgc tttccgagcc cgtctatgac aagaaatcca
     1081 caaacacatc agagctgcgg atttgccgaa ttaacaagga aagcgggccg tgcaccggtg
     1141 gcgaggagct ctacttgctc tgcgacaagg tgcagaaaga ggacatatca gtggtgttca
     1201 gcagggcctc ctgggaaggt cgggctgact tctcccaggc cgacgtgcac cgccagattg
     1261 ccattgtgtt caagacgccg ccctacgagg acctggagat tgtcgagccc gtgacagtca
     1321 acgtcttcct gcagcggctc accgatgggg tctgcagcga gccattgcct ttcacgtacc
     1381 tgcctcgcga ccatgacagc tacggcgtgg acaagaagcg gaaacggggg atgcccgacg
     1441 tccttgggga gctgaacagc tctgaccccc atggcatcga gagcaaacgg cggaagaaaa
     1501 agccggccat cctggaccac ttcctgccca accacggctc aggcccgttc ctcccgccgt
     1561 cagccctgct gccagaccct gacttcttct ctggcaccgt gtccctgccc ggcctggagc
     1621 cccctggcgg gcctgacctc ctggacgatg gctttgccta cgaccctacg gcccccacac
     1681 tcttcaccat gctggacctg ctgcccccgg caccgccaca cgctagcgct gttgtgtgca
     1741 gcggaggtgc cggggccgtg gttggggaga cccccggccc tgaaccactg acactggact
     1801 cgtaccaggc cccgggcccc ggggatggag gcaccgccag ccttgtgggc agcaacatgt
     1861 tccccaatca ttaccgcgag gcggcctttg ggggcggcct cctatccccg gggcctgaag
     1921 ccacgtaccc aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     1981 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     2041 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     2101 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga
     2161 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     2221 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     2281 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     2341 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     2401 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     2461 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     2521 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     2581 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     2641 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     2701 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     2761 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     2821 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     2881 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     2941 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     3001 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     3061 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     3121 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     3181 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     3241 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     3301 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     3361 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3421 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3481 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3541 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3601 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3661 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3721 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3781 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3841 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3901 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3961 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4021 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4081 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4141 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4201 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4261 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     4321 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     4381 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     4441 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     4501 ctgagccttt cgttttattt gatgcct
//
