LOCUS       sequence-16515-e         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Cashikar AG, Schirmer EC, Hattendorf DA, Glover JR, Ramakrishnan MS,
            Ware DM, Lindquist SL
  TITLE     Defining a pathway of communication from the C-terminal peptide 
            binding domain to the N-terminal ATPase domain in a AAA protein.
  JOURNAL   Mol Cell 2002 Apr;9(4):751-60.
  PUBMED    11983167
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell"; 
            date: "2002-04"; volume: "9(4)"; pages: "751-60"
COMMENT     Sequence Label: pJC45BS104 L553W
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..61
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             74..85
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttaactttaa gaaggagata taccatgggg ccatcatcat catcatcatc atcatcatca
       61 cagcagcggc catatcgaag gtcgtggatc cgggatgaac gaccaaacgc aatttacaga
      121 aagggctcta acgattttga cgttggctca aaaattggct tcggatcatc aacatccaca
      181 attacaacct atacatattc tagctgcctt cattgaaacg ccagaagatg gatcagtccc
      241 ttacctacag aatctaattg agaagggccg ttacgactat gatcttttca agaaagtggt
      301 taatagaaat ctagtaagaa ttcctcaaca gcaacctgca cctgcggaga taactccaag
      361 ttatgctttg gggaaagtcc ttcaagacgc tgctaagatt caaaaacaac agaaggactc
      421 atttatagcg caagaccata tattgtttgc tctattcaat gattcgtcta ttcagcaaat
      481 atttaaggaa gctcaagtag atattgaggc catcaagcaa caagctcttg aacttcgtgg
      541 taacactaga attgactctc gtggcgctga tacgaacaca cctttggaat atttatcaaa
      601 gtacgccatt gatatgactg agcaggctcg t
//
