LOCUS       sequence-165213-        1737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  luminescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Suzuki K, Kimura T, Shinoda H, Bai G, Daniels MJ, Arai Y, Nakano M, 
            Nagai T
  TITLE     Five colour variants of bright luminescent protein for real-time 
            multicolour bioimaging.
  JOURNAL   Nat Commun. 2016 Dec 14;7:13718. doi: 10.1038/ncomms13718.
  PUBMED    27966527
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-12-14"; volume: "7:13718. doi"; pages: " 
            10.1038/ncomms13718"
COMMENT     Sequence Label: GeNL(Ca2+)_60/pcDNA3
FEATURES             Location/Qualifiers
     source          1..1737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1336..1413
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 atggtgtcca agggcgaaga ggacaacatg gccagcctgc ctgccaccca cgagctgcac
       61 atcttcggca gcatcaacgg cgtggacttc gacatggtgg gacagggcac cggcaacccc
      121 aacgacggct acgaggaact gaacctgaag tccacaaagg gcgacctgca gttcagcccc
      181 tggattctgg tgccccacat cggctacggc ttccaccagt acctgcccta ccccgacggc
      241 atgagccctt tccaggccgc tatggtggat ggcagcggct accaggtgca ccggaccatg
      301 cagtttgagg acggcgccag cctgaccgtg aactaccggt acacatacga gggcagccac
      361 atcaagggcg aggcccaagt gaagggcaca ggctttccag ccgacggccc cgtgatgacc
      421 aatagcctga cagccgccga ctggtgcaga agcaagaaaa cctaccccaa tgacaagacc
      481 atcatcagca ccttcaagtg gtcctacacc accggcaatg gcaagcggta cagaagcacc
      541 gcccggacca cctacacctt cgccaaacct atggccgcca actacctgaa gaaccagcct
      601 atgtacgtgt tccgcaagac cgagctgaag cactccaaga cagaactgaa cttcaaagag
      661 tggcagaaag ccttcaccgg gtttgaagat ttcgttgggg actggcgaca gacagccggc
      721 tacaacctgg accaagtcct tgaacaggga ggtgtgtcca gtttgtttca gaatctcggg
      781 gtgtccgtaa ctccgatcca aaggattgtc ctgagcggtg aaaatgggct gaagatcgac
      841 atccatgtca tcatcccgta tgaaggtcca tggatgcatg accaactgac agaagagcag
      901 attgcagagt tcaaagaagc cttctcatta ttcgacaagg atggggacgg caccatcacc
      961 acaagggaac ttggcaccgt tatgaggtcg cttggacaaa acccaacgga agccgaattg
     1021 caggatatga tcaatgaagt cgatgctgat ggcaatggaa cgatttactt tcctgaattt
     1081 cttactatga tggctagaaa aatgaaggac acagacagcg aagaggaaat ccgagaagca
     1141 ttccgtgttt ttgacaagga tgggaacggc tacatcagcg ctgctgaatt acgtcacgtc
     1201 atgacaaacc tcggggtgaa gttaacagat gaagaagttg atgaaatgat aagggaagca
     1261 gatatcgatg gtgatggcca agtaaactat gaagagtttg aacaaatgat gacagcaaag
     1321 gggggaggtg gctccaagag gcgctggaag aaaaacttca ttgccgtcag cgctgccaac
     1381 cggttcaaga agatctccag ctccggggca ctggagctcc tgagcggcga ccaaatgggc
     1441 cagatcgaaa aaatttttaa ggtggtgtac cctgtggatg atcatcactt taaggtgatc
     1501 ctgcactatg gcacactggt aatcgacggg gttacgccga acatgatcga ctatttcgga
     1561 cggccgtatg aaggcatcgc cgtgttcgac ggcaaaaaga tcactgtaac agggaccctg
     1621 tggaacggca acaaaattat cgacgagcgc ctgatcaacc ccgacggctc cctgctgttc
     1681 cgagtaacca tcaacggagt gaccggctgg cggctgtgcg aacgcattct ggcgtaa
//
