LOCUS       sequence-165223-        3768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MLST8 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MLST8_WT
FEATURES             Location/Qualifiers
     source          1..3768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      35..623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     524..543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     777..794
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(953..980)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1072..1158)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1207..1229
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1221..1238
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1222..1238
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1254..1353
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2332..2431)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2448..2467)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2449..2467)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2472..2488)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2472..2488)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2919..3710
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
       61 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
      121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
      661 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      721 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
      781 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
      841 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      901 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
      961 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     1021 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     1081 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     1141 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga
     1201 tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccccaaataa
     1261 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
     1321 ttataatgcc aactttgtac aaaaaagttg gcatgaacac ctccccaggc acggtgggca
     1381 gtgacccggt catcctggcc actgcaggct acgaccacac cgtgcgcttc tggcaggccc
     1441 acagcggcat ctgcacccgg acggtgcagc accaggactc ccaggtgaat gccttggagg
     1501 tcacaccgga ccgcagcatg attgctgctg caggttacca gcacatccgc atgtatgatc
     1561 tcaactccaa taaccctaac cccatcatca gctacgacgg cgtcaacaag aacatcgcgt
     1621 ctgtgggctt ccacgaagac ggccgctgga tgtacacggg cggcgaggac tgcacagcca
     1681 ggatctggga cctcaggtcc cggaacctgc agtgccagcg gatcttccag gtgaacgcac
     1741 ccattaactg cgtgtgcctg caccccaacc aggcagagct catcgtgggt gaccagagcg
     1801 gggctatcca catctgggac ttgaaaacag accacaacga gcagctgatc cctgagcccg
     1861 aggtctccat cacgtccgcc cacatcgatc ccgacgccag ctacatggca gctgtcaata
     1921 gcaccggaaa ctgctatgtc tggaatctga cggggggcat tggtgacgag gtgacccagc
     1981 tcatccccaa gactaagatc cctgcccaca cgcgctacgc cctgcagtgt cgcttcagcc
     2041 ccgactccac gctcctcgcc acctgctcgg ctgatcagac gtgcaagatc tggaggacgt
     2101 ccaacttctc cctgatgacg gagctgagca tcaagagcgg caaccccggg gagtcctccc
     2161 gcggctggat gtggggctgc gccttctcgg gggactccca gtacatcgtc actgcttcct
     2221 cggacaacct ggcccggctc tggtgtgtgg agactggaga gatcaagaga gagtatggcg
     2281 gccaccagaa ggctgttgtc tgcctggcct tcaatgacag tgtgctgggc tacccaactt
     2341 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
     2401 tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt
     2461 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     2521 gttacattgc acaagataaa aatatatcat catgcctcct ctagaccagc caggacagaa
     2581 atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga
     2641 aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta
     2701 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     2761 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
     2821 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     2881 cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg
     2941 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     3001 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     3061 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     3121 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     3181 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     3241 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     3301 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     3361 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     3421 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     3481 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     3541 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     3601 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     3661 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc
     3721 acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgag
//
