LOCUS       sequence-165281-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli expression vector derived from pET32 with Gateway cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  TITLE     Olszewski lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET32a-DEST
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(124..248)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             301..318
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(371..389)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      385..432
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      469..897
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(556..575)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     766..787
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
       61 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
      121 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
      181 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
      241 aaagtggtga tatcggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag
      301 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg
      361 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
      421 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta
      481 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
      541 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
      601 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
      661 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
      721 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
      781 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
      841 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
      901 acaaaatatt aacg
//
