LOCUS       sequence-165317-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HA tagged ERK5 kinase domain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Buschbeck M, Eickhoff J, Sommer MN, Ullrich A
  TITLE     Phosphotyrosine-specific phosphatase PTP-SL regulates the ERK5 
            signaling pathway.
  JOURNAL   J Biol Chem. 2002 Aug 16;277(33):29503-9. Epub 2002 May 31.
  PUBMED    12042304
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-08-16"; volume: "277(33)"; pages: "29503-9. Epub
            2002 May 31"
COMMENT     Sequence Label: pcDNA3-HA-ERK5kin
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             79..105
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttccg ccaccatgta tgatgttcct gattatgcta gcctcccggg aggatccgcc
      121 gagcctctga aggaggaaga cggcgaggac ggctctgcgg agccccccgg gcccgtgaag
      181 gccgaacccg cccacaccgc tgcctctgta gcggccaaga acctggccct gcttaaagcc
      241 cgctccttcg atgtgacctt tgacgtgggc ggcgagtacg agatcatcga gaccataggc
      301 aacggggcct atggagtggt gtcctccgcc cgccgccgcc tcaccggcca gcaggtggcc
      361 atcaagaaga tccctaatgc tttcgatgtg gtgaccaatg ccaagcggac cctcagggag
      421 ctgaagatcc tcaagcactt caaacacgac aacatcatcg ccatcaagga catcctgagg
      481 cccaccgtgc cctatggcga attcaaatct gtctacgtgg tcctggacct gatggaaagc
      541 gacctgcacc agatcatcca ctcctcacag cccctcacac tggaacacgt gcgctacttc
      601 ctgtaccaac tgctgcgggg cctgaagtac atgcactcgg ctcaggtcat ccaccgtgac
      661 ctgaagccct ccaacctatt ggtgaatgag aactgtgagc tcaagattgg tgactttggt
      721 atggctcgtg gcctgtgcac ctcgcccgct gaacatcagt acttcatgac tgagtatgtg
      781 gccacgcgct ggtaccgtgc gcccgagctc atgctctctt tgcatgagta tacacaggct
      841 attgacctct ggtctgtggg ctgcatcttt ggtgagatgc tggcccggcg c
//
