LOCUS       sequence-165319-        3960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TGFB1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TGFB1_WT
FEATURES             Location/Qualifiers
     source          1..3960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(47..74)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(166..252)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     301..323
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     315..332
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     316..332
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    348..447
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1618..1717)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1734..1753)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1735..1753)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1758..1774)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1758..1774)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2205..2996
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3089..3677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3578..3597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3831..3848
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
       61 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
      121 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
      181 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
      241 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt
      301 cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt
      361 tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa
      421 tgccaacttt gtacaaaaaa gttggcatgc cgccctccgg gctgcggctg ctgctgctgc
      481 tgctaccgct gctgtggcta ctggtgctga cgcctggccg gccggccgcg ggactatcca
      541 cctgcaagac tatcgacatg gagctggtga agcggaagcg catcgaggcc atccgcggcc
      601 agatcctgtc caagctgcgg ctcgccagcc ccccgagcca gggggaggtg ccgcccggcc
      661 cgctgcccga ggccgtgctc gccctgtaca acagcacccg cgaccgggtg gccggggaga
      721 gtgcagaacc ggagcccgag cctgaggccg actactacgc caaggaggtc acccgcgtgc
      781 taatggtgga aacccacaac gaaatctatg acaagttcaa gcagagtaca cacagcatat
      841 atatgttctt caacacatca gagctccgag aagcggtacc tgaacccgtg ttgctctccc
      901 gggcagagct gcgtctgctg aggctcaagt taaaagtgga gcagcacgtg gagctgtacc
      961 agaaatacag caacaattcc tggcgatacc tcagcaaccg gctgctggca cccagcgact
     1021 cgccagagtg gttatctttt gatgtcaccg gagttgtgcg gcagtggttg agccgtggag
     1081 gggaaattga gggctttcgc cttagcgccc actgctcctg tgacagcagg gataacacac
     1141 tgcaagtgga catcaacggg ttcactaccg gccgccgagg tgacctggcc accattcatg
     1201 gcatgaaccg gcctttcctg cttctcatgg ccaccccgct ggagagggcc cagcatctgc
     1261 aaagctcccg gcaccgccga gccctggaca ccaactattg cttcagctcc acggagaaga
     1321 actgctgcgt gcggcagctg tacattgact tccgcaagga cctcggctgg aagtggatcc
     1381 acgagcccaa gggctaccat gccaacttct gcctcgggcc ctgcccctac atttggagcc
     1441 tggacacgca gtacagcaag gtcctggccc tgtacaacca gcataacccg ggcgcctcgg
     1501 cggcgccgtg ctgcgtgccg caggcgctgg agccgctgcc catcgtgtac tacgtgggcc
     1561 gcaagcccaa ggtggagcag ctgtccaaca tgatcgtgcg ctcctgcaag tgcagctgcc
     1621 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     1681 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     1741 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     1801 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     1861 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     1921 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     1981 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     2041 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     2101 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     2161 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     2221 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     2281 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     2341 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     2401 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     2461 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     2521 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     2581 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     2641 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     2701 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     2761 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     2821 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     2881 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     2941 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     3001 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     3061 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3121 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3181 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3241 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3301 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3361 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3421 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3481 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3541 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3601 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3661 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3721 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3781 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3841 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3901 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
//
