LOCUS       sequence-165388-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Introduces DSB at MFE1 locus in Y. lipolytica.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Schwartz C, Shabbir-Hussain M, Frogue K, Blenner M, Wheeldon I
  TITLE     Standardized markerless gene integration for pathway engineering in 
            Yarrowia lipolytica.
  JOURNAL   ACS Synth Biol. 2016 Dec 19.
  PUBMED    27989123
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Dec 19."
COMMENT     Sequence Label: pCRISPRyl_MFE1
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        518..593
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     643..661
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtccccag
       61 ttgcaaaagt tgacacaact ctagatctgc ttccaaatat agaatcataa caagggttag
      121 ggtgtgatta tataatattg gtcttaattg atgtgctagg gctttaaaag ttggttaaaa
      181 taacgctcta atgccttttt aatatattgt ctttttcaaa atctcaaatc ggacacttct
      241 tcgtgtatga gactccattt tttggctccg tcacgtgata tgtattatca gctatagtgg
      301 tgtaaacaaa gttttttact agctgtaatg gcattttgtc ggagtggtaa atcgccttct
      361 tgttgtgcgt tcgagttctg gactctgcac tgggctactt tgaaaaatac ctctaatgcg
      421 ccgatggttt agtggtaaaa tccatcgttg ccatcgatgg gcccccggtt cgattccggg
      481 tcggcgcagg ttgacgtagt ctcaccgaaa ccaaccagtt ttagagctag aaatagcaag
      541 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
      601 tacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
      661 cccagatcct ctagagtcga agcggccgct atgtctgata aaaggatgta acataggcaa
      721 gctgctcgtg agtgttgagt acgaacctta gatccaaatc acccgcaccc acggatatac
      781 ttgcttgaat atacagtagt atgcggccgc ttcgacacca tatcatataa aactaacaat
      841 gcattgaatt aatacgaaga ctacccgttg ctatctccac accgttatct ccacggtcca
      901 aaggctgctc aatgtgctgc atac
//
