LOCUS       sequence-165422-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  [Edit] Dual Fluorescent endogenous miRNA sensor. To insert the 
            complementary region at the 3'UTR of CFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Lemus-Diaz N, Boker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M,
            Gruber J
  TITLE     Dissecting miRNA gene repression on single cell level with an 
            advanced fluorescent reporter system.
  JOURNAL   Sci Rep. 2017 Mar 24;7:45197. doi: 10.1038/srep45197.
  PUBMED    28338079
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-24"; volume: "7:45197. doi"; pages: " 
            10.1038/srep45197"
COMMENT     Sequence Label: p.UTA.2.0 Empty
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(80..793,794..796,797..799))
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(125..146)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     474..493
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     733..754
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(807..825)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(868..887)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          870..1002
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 atgtaatgaa aataaagata ttttattgcg gccagcggcc gctctaggtt taaacgaatt
       61 cccgggctcg agcgatcgct tacttgtaca gctcgtccat gccgagagtg atcccggcgg
      121 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
      181 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
      241 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
      301 tgaagtgggc cttgatgccg ttcttctgct tgtcggcggt gatatagacg ttgtggctga
      361 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
      421 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
      481 agttgccgtc gtccttgaag aagatggtac gctcctggac gtagccttcg ggcatggcgg
      541 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgcccc
      601 aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
      661 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaacc
      721 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
      781 cctcgccctt gctcaccatt ggctagccta tagtgagtcg tattaagtac tctagcctta
      841 agagctgtaa ttgaactggg agtggacacc tgtggagaga aaggcaaagt ggatgtcagt
      901 aagaccaata ggtgcctatc agaaacgcaa gagtcttctc tgtctcgaca agcccagttt
      961 ctattggtct ccttaaacct gtcttgtaac cttgatactt acctgcccag tgcctcacga
//
