LOCUS       sequence-165548-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral construct to express dCas9 fused with an inactive Dnmt3a 
            catalytic domain (E664A, E756A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, 
            Young RA, Jaenisch R
  TITLE     Editing DNA Methylation in the Mammalian Genome.
  JOURNAL   Cell. 2016 Sep 22;167(1):233-247.e17. doi: 
            10.1016/j.cell.2016.08.056.
  PUBMED    27662091
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.08"; journalName: "Cell"; date: "2016-09-22- 
            22"; volume: "167"; issue: "1"; pages: "233-247.e17"
COMMENT     Sequence Label: Fuw-dCas9-Dnmt3a_IM
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..76
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             80..100
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             104..124
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actttacgaa actcggattg acctctcaca gctcggaggg gatgagggag ctgatccaaa
       61 aaagaagaga aaggtagacc caaagaaaaa acgaaaagta gatccgaaaa agaagaggaa
      121 ggtgggatcc aaccacgacc aggaatttga ccctccaaag gtttacccac ctgtcccagc
      181 tgagaagagg aagcccatcc gggtgctgtc tctctttgat ggaatcgcta cagggctcct
      241 ggtgctgaag gacttgggca ttcaggtgga ccgctacatt gcctcggccg tgtgtgagga
      301 ctccatcacg gtgggcatgg tgcggcacca ggggaagatc atgtacgtcg gggacgtccg
      361 cagcgtcaca cagaagcata tccaggagtg gggcccattc gatctggtga ttgggggcag
      421 tccctgcaat gacctctcca tcgtcaaccc tgctcgcaag ggcctctacg agggcactgg
      481 ccggctcttc tttgagttct accgcctcct gcatgatgcg cggcccaagg agggagatga
      541 tcgccccttc ttctggctct ttgccaatgt ggtggccatg ggcgttagtg acaagaggga
      601 catctcgcga tttctcgagt ccaaccctgt gatgattgat gccaaagaag tgtcagctgc
      661 acacagggcc cgctacttct ggggtaacct tcccggtatg aacaggccgt tggcatccac
      721 tgtgaatgat aagctggagc tgcaggagtg tctggagcat ggcaggatag ccaagttcag
      781 caaagtgagg accattacta cgaggtcaaa ctccataaag cagggcaaag accagcattt
      841 tcctgtcttc atgaatgaga aagagga
//
