LOCUS       sequence-165556-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral construct to express dCas9 fused with Dnmt3a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, 
            Young RA, Jaenisch R
  TITLE     Editing DNA Methylation in the Mammalian Genome.
  JOURNAL   Cell. 2016 Sep 22;167(1):233-247.e17. doi: 
            10.1016/j.cell.2016.08.056.
  PUBMED    27662091
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.08"; journalName: "Cell"; date: "2016-09-22- 
            22"; volume: "167"; issue: "1"; pages: "233-247.e17"
COMMENT     Sequence Label: Fuw-dCas9-Dnmt3a
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..67
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             71..91
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             95..115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aactcggatt gacctctcac agctcggagg ggatgaggga gctgatccaa aaaagaagag
       61 aaaggtagac ccaaagaaaa aacgaaaagt agatccgaaa aagaagagga aggtgggatc
      121 catgcccgcc atgccctcca gcggccccgg ggacaccagc agctctgctg cggagcggga
      181 ggaggaccga aaggacggag aggagcagga ggagccgcgt ggcaaggagg agcgccaaga
      241 gcccagcacc acggcacgga aggtggggcg gcctgggagg aagcgcaagc accccccggt
      301 ggaaagcggt gacacgccaa aggaccctgc ggtgatctcc aagtccccat ccatggccca
      361 ggactcaggc gcctcagagc tattacccaa tggggacttg gagaagcgga gtgagcccca
      421 gccagaggag gggagccctg ctggggggca gaagggcggg gccccagcag agggagaggg
      481 tgcagctgag accctgcctg aagcctcaag agcagtggaa aatggctgct gcacccccaa
      541 ggagggccga ggagcccctg cagaagcggg caaagaacag aaggagacca acatcgaatc
      601 catgaaaatg gagggctccc ggggccggct gcggggtggc ttgggctggg agtccagcct
      661 ccgtcagcgg cccatgccga ggctcacctt ccaggcgggg gacccctact acatcagcaa
      721 gcgcaagcgg gacgagtggc tggcacgctg gaaaagggag gctgagaaga aagccaaggt
      781 cattgcagga atgaatgctg tggaagaaaa ccaggggccc ggggagtctc agaaggtgga
      841 ggaggccagc cctcctgctg tgcagcagcc cactgacccc gcatccccca ctgtggctac
      901 cacgcctgag cccgtggggt ccgatg
//
