LOCUS       sequence-165614-         460 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CyRFP1 fluorescent protein in an AAV backbone, FLEX 
            version (Cre dependent).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
  TITLE     Simultaneous dual-color fluorescence lifetime imaging with novel 
            red-shifted fluorescent proteins.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
  PUBMED    27798609
REFERENCE   2  (bases 1 to 460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046."
COMMENT     Sequence Label: AAV-FLEX-CAG-CyRFP1
FEATURES             Location/Qualifiers
     source          1..460
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(21..54)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(105..138)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cctctagagt cgactccgga ataacttcgt ataggatact ttatacgaag ttatgcagaa
       61 tggtagctgg attgtagctg ctattagcaa tatgaaacct cttaataact tcgtatagca
      121 tacattatac gaagttatgg cgcgccataa tatcacttgt acagctcgtc catgcctccg
      181 cccaggttgg agtatctggc cacagcgtgc tcgtactgct cgacgtaggt ctcgttgtcg
      241 gcctccttga ttctttccag tctgcggtcc acgtagtgga cgccgggcat cttcacgggt
      301 ttcttggact tgtatgtggt cttgaagttg acgtgcaggt ggcccccgcc cacgagcttc
      361 agggccttgt cgcatctgcc ttccaggccg ccgtcagcgg ggtacatggt ctcggtgctg
      421 ggctcccagc ccagtgtttt cttctgcatc acggggccgt
//
