LOCUS       sequence-165616-         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CyRFP1 fluorescent protein in an AAV backbone, FLEX 
            version (Cre dependent).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
  TITLE     Simultaneous dual-color fluorescence lifetime imaging with novel 
            red-shifted fluorescent proteins.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
  PUBMED    27798609
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046."
COMMENT     Sequence Label: AAV-FLEX-CAG-CyRFP1
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(48..81)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(132..165)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      179..188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattcata acttcgtata
       61 gcatacatta tacgaagtta ttctttgcac cattctaaag aataacagtg ataatttctg
      121 ggttaaggca aataacttcg tataggatac tttatacgaa gttatgctag cttcggtcgc
      181 caccatggtg agcaagggcg aggagctgat caaggagaac atgagaagca agctgtacct
      241 ggaaggcagc gtgaacggcc accagttcaa gtgcacccac gaaggggagg gcaagcccta
      301 cgagggcaag cagaccaaca ggatcaaggt ggtggaggga ggccccctgc cgttcgcatt
      361 cgacatcctg gccaccatgt ttatgtacgg gagcaaggtg ttcatcaagt accccgccga
      421 cctccccgat tattttaagc agtccttccc tgagggcttc acatgggaga gagtcatggt
      481 gttcgaagac gggggcgtgc tgaccgccac ccaggacacc agcctccagg acggcgagct
      541 catctacaac gtcaagctca gaggggtgaa cttcccagcc aacggccccg tgatgcagaa
      601 gaaaacactg ggctgggagc ccagcaccga gaccatgtac cccgctgacg gcggcctgga
      661 aggcagatgc gacaaggccc tgaagctcgt gggcgggggc cacctgcacg
//
