LOCUS       sequence-165623-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fusion protein of RhoA, FRET donor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
  TITLE     Simultaneous dual-color fluorescence lifetime imaging with novel 
            red-shifted fluorescent proteins.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
  PUBMED    27798609
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046."
COMMENT     Sequence Label: mCyRFP1-RhoA
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(137..718)
                     /codon_start=1
                     /product="human Rho family small GTPase RhoA"
                     /label=RhoA
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
ORIGIN
        1 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
       61 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagttatc
      121 tagatccggt ggatcctcac aagacaaggc acccagattt tttcttccca cgtctagctt
      181 gcagagcagc tctcgtagcc atttcaaaaa cctctctcac tccatctttg gtctttgctg
      241 aacactccat gtacccaaaa gcgccaatcc tgtttgccat atctctgcct tcttcaggtt
      301 tcaccggctc ctgcttcatc ttggctagct cccgccttgt gtgctcatca ttccgaagat
      361 ccttcttgtt cccaaccagg atgatgggca cgttgggaca gaaatgcttg acttctgggg
      421 tccacttttc tgggatgttt tctaaactat cagggctgtc gatggaaaaa cacatcagta
      481 taacatcggt atctgggtag gagaggggcc tcaggcgatc ataatcttcc tgcccagctg
      541 tgtcccacaa agccaactct acctgctttc catccacctc gatatctgcc acatagttct
      601 caaacactgt gggcacatac acctctggga actggtcctt gctgaagact atgagcaagc
      661 atgtctttcc acaggctcca tcaccaacaa tcaccagttt cttccggatg gcagccatcc
      721 ctcgagatct gagtccggac ttgtacagct cgtccatgcc tccgcccagg ttggagtatc
      781 tggccacagc gtgctcgtac tgctcgacgt aggtctcgtt gtcggcctcc ttgattctt
//
