LOCUS       sequence-165627-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ABA-inducible KRAB-Sp dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV4...
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             620..805
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
ORIGIN
        1 gggtgggggc gcgccaactc aagacgaatt cacccaactc tcccaatcaa tcgccgagtt
       61 ccacacgtac caactcggta acggccgttg ctcatctctc ctagctcagc gaatccacgc
      121 gccgccggaa acagtatggt ccgtggtgag acgtttcgat aggccacaga tttacaaaca
      181 cttcatcaaa agctgtaacg tgagtgaaga tttcgagatg cgagtgggat gcacgcgcga
      241 cgtgaacgtg ataagtggat taccggcgaa tacgtctcga gagagattag atctgttgga
      301 cgatgatcgg agagtgactg ggtttagtat aaccggtggt gaacataggc tgaggaatta
      361 taaatcggtt acgacggttc atagatttga gaaagaagaa gaagaagaaa ggatctggac
      421 cgttgttttg gaatcttatg ttgttgatgt accggaaggt aattcggagg aagatacgag
      481 attgtttgct gatacggtta ttagattgaa tcttcagaaa cttgcttcga tcactgaagc
      541 tatgaacgaa ttctcaagcg ctgcaggtac tagtggcggt ggcggaggga tggatgctaa
      601 gtcactaact gcctggtccc ggacactggt gaccttcaag gatgtatttg tggacttcac
      661 cagggaggag tggaagctgc tggacactgc tcagcagatc gtgtacagaa atgtgatgct
      721 ggagaactat aagaacctgg tttccttggg ttatcagctt actaagccag atgtgatcct
      781 ccggttggag aagggagaag agccctagat ttaaataatt cgcccctctc cctccccccc
      841 ccctaacgtt actggccgaa gccgcttgga ataaggccgg
//
