LOCUS       sequence-165644-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET donor for dynamically measuring TrkB activity in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Harward SC, Hedrick NG, Hall CE, Parra-Bueno P, Milner TA, Pan E, 
            Laviv T, Hempstead BL, Yasuda R, McNamara JO
  TITLE     Autocrine BDNF-TrkB signalling within a single dendritic spine.
  JOURNAL   Nature. 2016 Sep 28;538(7623):99-103. doi: 10.1038/nature19766.
  PUBMED    27680698
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-09-28- 28"; volume: "538"; issue: "7623"; 
            pages: "99-103"
COMMENT     Sequence Label: TrkB mEGFP
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..237
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      279..288
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
       61 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
      121 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
      181 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
      241 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggtggcga ccggtggatc
      301 cgcgcctagg atatccaggt agacgggaga tgccttggcc aagttctgaa ggagggtgtg
      361 gatgctcttg atgttcttcc gggtgtgtgg ttcccgctgc cagcatccga gcatgagctc
      421 atacacctcc tggggacagg ttcgaggccg ctgaaggact cttccctggg tgatgcactc
      481 tatcacctca ttgttcgata gctgatacca gggctgcttg ccgtaggtga agatctccca
      541 caacacaacg cccaggctcc agacgtcgct ctcggtggtg aatttcctat acatgatgct
      601 ctctggaggc atccatcgga tgggcaacat tgtgtggcca ccgacccgat agtagtcggt
      661 gctgtacaca tctcgggaca tcccaaagtc cccaattttc accagcaggt tctctcccac
      721 caggcagttc cgggtggcca ggtcacggtg cacaaagtgt tgggacgcca ggtagaccat
      781 acctgctgcg atttgctgag cgatgtgcag catctgcgac tgcgtcagct ctgtgggcgg
      841 gttacc
//
