LOCUS       sequence-165658-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GA-inducible VPR-Sa dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE
            PGK-G...
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagaattcgc caccatggct gcgagcgatg aagttaatct tattgagagc agaacagtgg
       61 ttcctctcaa tacatgggtt ttaatatcca acttcaaagt agcctacaat atccttcgtc
      121 gccctgatgg aacctttaac cgacacttag ctgagtatct agaccgtaaa gtcactgcaa
      181 acgccaatcc ggttgatggg gttttctcgt tcgatgtctt gattgatcgc aggatcaatc
      241 ttctaagcag agtctataga ccagcttatg cagatcaaga gcaacctcct agtattttag
      301 atctcgagaa gcctgttgat ggcgacattg tccctgttat attgttcttc catggaggta
      361 gctttgctca ttcttctgca aacagtgcca tctacgatac tctttgtcgc aggcttgttg
      421 gtttgtgcaa gtgtgttgtt gtctctgtga attatcggcg tgcaccagag aatccatacc
      481 cttgtgctta tgatgatggt tggattgctc ttaattgggt taactcgaga tcttggctta
      541 aatccaagaa agactcaaag gtccatattt tcttggctgg tgatagctct ggaggtaaca
      601 tcgcgcataa tgtggcttta agagcgggtg aatcgggaat cgatgttttg gggaacattc
      661 tgctgaatcc tatgtttggt gggaatgaga gaacggagtc tgagaaaagt ttggatggga
      721 aatactttgt gacggttaga gaccgcgatt ggtactggaa agcgttttta cccgagggag
      781 aagatagaga gcatccagcg tgtaatccgt ttagcccgag agggaaaagc ttagaaggag
      841 tgagtttccc caagagtctt gtggttgtcg cgggtttgga tttgattaga gattggcagt
      901 tggcatac
//
