LOCUS       sequence-165681-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the dehydrogenase-deficient mutant 
            DLD-H450A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, 
            Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, 
            Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster
            LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson 
            J, Babu M
  TITLE     A Map of Human Mitochondrial Protein Interactions Linked to 
            Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and 
            NF-kappaB Signaling.
  JOURNAL   Cell Syst. 2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010.
  PUBMED    29128334
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010."
COMMENT     Sequence Label: pPHAGE_DLD-H450A-C-TAP
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(148..174)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(148..174)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(190..213)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    245..269
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcgtcaaga agacagggcc aggtttccgg gccctcacat tgccaaaaga cggcaatatg
       61 gtggaaaata acatatagac aaacgcacac cggccttatt ccaagcggct tcggccagta
      121 acgttagggg gggggggcgg aattctaggc gtagtcgggc acgtcgtagg ggtatcctcc
      181 agcggccgac ttgtcatcgt cgtccttgta gtcctcgagt tcgaaccgcg ggccctctag
      241 atcaaccact ttgtacaaga aagttgggca aaagttgatt gatttgccaa atgacgcagc
      301 aagatttgct tctctaaaag cttctgataa ggtcggatgt gctgcacaga ctctagctat
      361 atcttcacag gatgctccat attccaaagc aagagcagct tcatttacca tttctccagc
      421 acctggtcca agaatatgtg ctcccagtac tctgtctgtc gatttctgcc caaggatctt
      481 caccatgcca tctgtgtcag catttgtctt agctctgctg ttagcagcaa atgggaattt
      541 cccaactttg tactcaatac cctcttcttt caactgctct tctgatttgc caacccaagc
      601 aacttcaggg tgtgtgtaaa tcactgatgg cacacaattg tagtcaatgt gcacagcacc
      661 accagccatt ccttcaacac agataatgcc ttcatcctct gctttgtgag ccagcattgg
      721 accagcaact acatcaccaa tggcatagat atttggaatt ttagtttgaa atctggtatt
      781 gactggaatt ctacctctgg gatctagttc aattcccagc tcttctagtc ccaaattctt
      841 agtaaagggt cgtcggccaa tgcaaaccaa gagtacatca caagtgataa cttcagcttt
      901 accaccagaa gcagcttca
//
