LOCUS       sequence-165720-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses direct fusion VPR-Sa dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-Sad...
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             62..211
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             218..238
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttgtcg acgccaccat
       61 ggatgcttta gacgattttg acttagatat gcttggttca gacgcgttag acgacttcga
      121 cctagacatg ttaggctcag atgcattgga cgacttcgat ttagatatgt tgggctccga
      181 tgccctagat gactttgatc tagatatgct aggtagtccc aaaaagaaga ggaaagtggg
      241 atcccagtat ctgcccgaca cagatgatag acaccgaatc gaagagaaac gcaagcgaac
      301 gtatgaaacc ttcaaatcga tcatgaagaa atcgcccttc tcgggtccga ccgatcccag
      361 gcccccaccg agaaggattg cggtcccgtc ccgctcgtcg gccagcgtgc cgaagcctgc
      421 gccgcagccc taccccttca cgtcgagcct gagcacaatc aattatgacg agttcccgac
      481 gatggtgttc ccctcgggac aaatctcaca agcctcggcg ctcgcaccag cgcctcccca
      541 agtccttccg caagcgcctg ccccagcgcc tgcaccggca atggtgtccg ccctcgcaca
      601 ggcccctgcg cccgtccccg tgctcgcgcc tggaccgccc caggcggtcg ctccaccggc
      661 tccgaagccg acgcaggccg gagagggaac actctccgaa gcacttcttc aactccagtt
      721 tgatgacgag gatcttggag cactccttgg aaactcgaca gaccctgcgg tgtttaccga
      781 cctcgcgtca gtagataact ccgaatttca gcagcttttg aaccagggta tcccggtcgc
      841 gccacataca acggagccca tgttgatgga ataccccgaa gcaatcacga gacttgtgac
      901 gggagcgcag cggcctcccg atcccgcacc cgcacctttg ggggcacctg gcctccctaa
      961 cggacttttg agcggcgacg aggatttctc ctccatcgcc gatatggatt t
//
