LOCUS       sequence-16573-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Glover JR, Kowal AS, Schirmer EC, Patino MM, Liu JJ, Lindquist S
  TITLE     Self-seeded fibers formed by Sup35, the protein determinant of 
            [PSI+], a heritable prion-like factor of S. cerevisiae.
  JOURNAL   Cell 1997 May 30;89(5):811-9.
  PUBMED    9182769
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "1997-05-30"; volume: "89(5)"; pages: "811-9"
COMMENT     Sequence Label: pRSCupSup35GFP
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..38
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     23..39
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        460..694
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
ORIGIN
        1 aagctggtac cgggcccccc ctcgaggtcg acggtatcga taagcttgat atcgaatttc
       61 ccattaccga catttgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg
      121 catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg
      181 tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct
      241 acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac
      301 ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag ctaaaacact
      361 tttgtattat ttttcctcat atatgtgtat aggtttatac ggatgattta attattactt
      421 caccaccctt tatttcaggc tgatatctta gccttgttac tagttagaaa aagacatttt
      481 tgctgtcagt cactgtcaag agattctttt gctggcattt cttctagaag caaaaagagc
      541 gatgcgtctt ttccgctgaa ccgttccagc aaaaaagact accaacgcaa tatggattgt
      601 cagaatcata taaaagagaa gcaaataact ccttgtcttg tatcaattgc attataatat
      661 cttcttgtta gtgcaatatc atatagaagt catcgaaata gatattaaga aaaacaaact
      721 gtacaatcaa tcaatcaatc aggatccatg tcggattcaa accaaggcaa caatcagcaa
      781 aactaccagc aatacagcca gaacggtaac caacaacaag gtaacaacag ataccaaggt
      841 tatcaagctt acaatgctca agcccaacct gca
//
