LOCUS       sequence-165738-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses direct fusion VPR-Sa dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-Sad...
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   71..133
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             complement(323..370)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 caattaaccc tcactaaagg gaacaaaagc tggattaatt aagcggccgc atacgcgtat
       61 actagattaa ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgcgta
      121 aaattgacgc atgtgtttta tcggtctgta tatcgaggtt tatttattaa tttgaataga
      181 tattaagttt tattatattt acacttacat actaataata aattcaacaa acaatttatt
      241 tatgtttatt tatttattaa aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa
      301 aacttttaac tagctcgagt tactttttct tttttgcctg gccggccttt ttcgtggccg
      361 ccggcctttt gccctttttg atgatctgag ggtgcttctt agatttcact tcatacaggt
      421 tgcccagaat gtctgtgctg tacttcttaa tgctctgggt cttggaggcg attgtcttaa
      481 tgatcctggg gggcctcttg tcgttcatgt tttccaggta ctcgcggtag gtgatgtcga
      541 tcatgttcac ttcgatccgg ttcagcaggt cgttgttcac gccgatcact ctatacagct
      601 cgccgttgat cttgatcaga tcgttgttgt agaaggaggc gataaactcg gcctggttgc
      661 tgatcttctt cagcttctta gcttcctcat agcacttgct attcacttcg tagtagtttt
      721 cttttttgat cacatccaga ttcttcacgg tcacgaactt gtacacgcca ttgtccaggt
      781 acacgtcgaa tctgtagggc ttcagggaca gcttcacgac cttgtttctg ctgttggggt
      841 agtcgtcggt gatgtccaga tgggcgttca gtttgttgcc gtaatactta atcttcttga
      901 tcacggggcc gttgtccttt ttggagtact tggtcaggta gttcccggtt tcctcgtagt
      961 acttgtacag gggattcttc tc
//
