LOCUS       sequence-165814-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd-generation lentiviral destination vector for gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: pEpic_Lite
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..69
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     53..69
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    89..212
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(253..558)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(355..374)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aaattcaaaa ttttatcgat cacgagacta gcctcgagaa gcttgattcg agcaggaaac
       61 agctatgacc atgattacgc caagctatca actttgtata gaaaagttga acgagaaacg
      121 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      181 tactgtaaaa cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag
      241 ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc
      301 gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac tggccatatc
      361 ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacgggg
      421 gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt
      481 gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt
      541 gtaaacctta aactgcattt caccagcccc tgttctcgtc ggcaaaggag ccgttcattt
      601 caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac
      661 gcagacgacg ggcttcattc tgcatggttg tgcttaccga accggagata ttgacatcat
      721 atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat
      781 agcatacctc tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa
//
