LOCUS       sequence-165821-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Sp sgCD95-1 gRNA with an EGFP fluorescent marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFP
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(205..238)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     242..262
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     enhancer        556..859
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ccgctctaga gatccgacgc gccatctcta ggcccgcgcc ggccccctcg cacggacttg
       61 tgggagaagc tcggctactc ccctgccccg gttaatttgc atataatatt tcctagtaac
      121 tatagaggct taatgtgcga taaaagacag ataatctgtt ctttttaata ctagctacat
      181 tttacatgat aggcttggat ttctataact tcgtatagca tacattatac gaagttataa
      241 acagcacaaa aggaaactca ccctaactgt aaagtaattg tgtgttttga gactataagt
      301 atcccttgga gaaccacctt gttggtacag cagaagcctt tagaagttta agagctatgc
      361 tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa aaagtggcac
      421 cgagtcggtg ctttttttct cgagtactag gatccattag gcggccgcgt ggataaccgt
      481 attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc
      541 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      601 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      661 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      721 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      781 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      841 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      901 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      961 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac t
//
