LOCUS       sequence-165822-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integrating plasmid, K. polysporus Dcr1 under TEF promoter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Nakanishi K, Weinberg DE, Bartel DP, Patel DJ
  TITLE     Structure of yeast Argonaute with guide RNA.
  JOURNAL   Nature. 2012 Jun 20;486(7403):368-74. doi: 10.1038/nature11211.
  PUBMED    22722195
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-06-20- 20"; volume: "486"; issue: "7403"; 
            pages: "368-74"
COMMENT     Sequence Label: pRS405-LEU2-TEF-KpolDcr1v2
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..77
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     61..77
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             239..250
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ttacaacttt ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt
       61 tctagaacta gtggatccat gtctgatcaa gtttatgttc cgccaacatc agcaccaatg
      121 agcaacgagt taaaagttag agaattttat agactccaca atgcttgtgt gaaattgaaa
      181 gagtctatta aattgattta tgaaaaccca ttggtaactg accaaaatgt tttgaatttg
      241 ggaactgcag aaaatactat tgattataca attttaaaca cacctacact aaatgttgct
      301 aaaactttac tgggcaatag atattcatta gatctaatag atctttttca atctcatgat
      361 ttcaaagatt caaacactga tgtggatatg tttattaaat atcctgtggt ttatgatgaa
      421 aatttggaaa atttagcatt tatgcataaa agttttccaa atatgaatgc acatctaaat
      481 gatgctcaaa agactcaatt atctaatgaa aggttagaat ttttgggtga tagttggcta
      541 ggtgctttag tttcatatat tgtatacacc agatttcctt ctgcaaatga aggtatgctt
      601 tctcaaatga aagaatcaat agtaaataat aataatctat ttgattggag tacaaaatta
      661 aacttcacca agagattaca aggtaatatc gctacaccta ctagagtggt caaagataaa
      721 atgtctaaac gttatgctga ttgtgttgaa gcctatattg gtgcattggt tattgataga
      781 tttggcactg agtttttgga tatcaaggaa tggttagaag aactatcaga aaaaaaattg
      841 gccaaatcga gccaaatggt gataaaggaa ccattaaata aaaatgctaa aaatgaatta
      901 gccgaat
//
