LOCUS       sequence-165854-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of recombinant human mature (delta38) METTL20 wt with 
            N-terminal 6xHis-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Malecki J, Ho AY, Moen A, Dahl HA, Falnes PO
  TITLE     Human METTL20 is a mitochondrial lysine methyltransferase that 
            targets the beta subunit of electron transfer flavoprotein (ETFbeta)
            and modulates its activity.
  JOURNAL   J Biol Chem. 2015 Jan 2;290(1):423-34. doi: 10.1074/jbc.M114.614115.
            Epub 2014 Nov 21.
  PUBMED    25416781
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.614115"; journalName: "J Biol Chem"; date: 
            "2015-01-2- 2"; volume: "290"; issue: "1"; pages: "423-34"
COMMENT     Sequence Label: pF708-pET28a-Hs-delta38-METTL20-NHis wt
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             829..846
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(899..917)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggtgc cgcgcggcag ccatatggct ggaagctttt
      121 tggaccctga gataaaggct ttcctggagg agaacactga agtcaccagc agtggtagcc
      181 tcacccctga aatccagttg cggcttttga cccccagatg caaattctgg tgggagagag
      241 ctgacctgtg gccccacagt gatccttact gggcaatcta ctggccagga ggccaagccc
      301 tgtctaggta tcttttggat aatcctgatg ttgtcagagg aaaatctgta ttagatcttg
      361 ggagtggatg tggagctaca gctattgctg ctaagatgag tggggcatca aggatcttgg
      421 ccaatgacat agaccctatt gcaggaatgg ctattacact aaattgtgaa ttgaacagac
      481 tgaatccttt tcctatttta atccaaaaca ttttgaattt ggaacaagat aagtgggacc
      541 ttgttgttct tggcgatatg ttttatgatg aagaccttgc agatagtctt catcagtggc
      601 tgaagaagtg cttctggacc tatagaactc gagtactgat tggtgaccct gggcggcccc
      661 agttcagtgg acacagcatt cagcatcacc tgcacaaagt ggtagaatat tcacttttgg
      721 agtctactag gcaggaaaac agtggactga caacaagcac agtgtggggt tttcagcctt
      781 gaggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac
      841 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      901 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttt
//
