LOCUS       sequence-165858-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of recombinant human mature (delta38) METTL20 wt with 
            N-terminal 6xHis-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Malecki J, Ho AY, Moen A, Dahl HA, Falnes PO
  TITLE     Human METTL20 is a mitochondrial lysine methyltransferase that 
            targets the beta subunit of electron transfer flavoprotein (ETFbeta)
            and modulates its activity.
  JOURNAL   J Biol Chem. 2015 Jan 2;290(1):423-34. doi: 10.1074/jbc.M114.614115.
            Epub 2014 Nov 21.
  PUBMED    25416781
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.614115"; journalName: "J Biol Chem"; date: 
            "2015-01-2- 2"; volume: "290"; issue: "1"; pages: "423-34"
COMMENT     Sequence Label: pF708-pET28a-Hs-delta38-METTL20-NHis wt
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(775..792)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(802..819)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattcgga tcctcaaggc tgaaaacccc acactgtgct
      121 tgttgtcagt ccactgtttt cctgcctagt agactccaaa agtgaatatt ctaccacttt
      181 gtgcaggtga tgctgaatgc tgtgtccact gaactggggc cgcccagggt caccaatcag
      241 tactcgagtt ctataggtcc agaagcactt cttcagccac tgatgaagac tatctgcaag
      301 gtcttcatca taaaacatat cgccaagaac aacaaggtcc cacttatctt gttccaaatt
      361 caaaatgttt tggattaaaa taggaaaagg attcagtctg ttcaattcac aatttagtgt
      421 aatagccatt cctgcaatag ggtctatgtc attggccaag atccttgatg ccccactcat
      481 cttagcagca atagctgtag ctccacatcc actcccaaga tctaatacag attttcctct
      541 gacaacatca ggattatcca aaagatacct agacagggct tggcctcctg gccagtagat
      601 tgcccagtaa ggatcactgt ggggccacag gtcagctctc tcccaccaga atttgcatct
      661 gggggtcaaa agccgcaact ggatttcagg ggtgaggcta ccactgctgg tgacttcagt
      721 gttctcctcc aggaaagcct ttatctcagg gtccaaaaag cttccagcca tatggctgcc
      781 gcgcggcacc aggccgctgc tgtgatgatg atgatgatgg ctgctgccca tggtatatc
//
