LOCUS       sequence-165880-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of recombinant human full-length METTL20 wt with 
            N-terminal 6xHis-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Malecki J, Ho AY, Moen A, Dahl HA, Falnes PO
  TITLE     Human METTL20 is a mitochondrial lysine methyltransferase that 
            targets the beta subunit of electron transfer flavoprotein (ETFbeta)
            and modulates its activity.
  JOURNAL   J Biol Chem. 2015 Jan 2;290(1):423-34. doi: 10.1074/jbc.M114.614115.
            Epub 2014 Nov 21.
  PUBMED    25416781
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.614115"; journalName: "J Biol Chem"; date: 
            "2015-01-2- 2"; volume: "290"; issue: "1"; pages: "423-34"
COMMENT     Sequence Label: pF714-pET28a-Hs-FL-METTL20-NHis wt
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..97
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca
       61 tcatcatcac agcagcggcc tggtgccgcg cggcagccat atggctttga gtctaggttg
      121 gaaagcacac aggaaccact gtggtctcct cttgcaggct ctgcgaagca gtggtcttct
      181 cttgtttccc tgtggccagt gtccctggag aggagctgga agctttttgg accctgagat
      241 aaaggctttc ctggaggaga acactgaagt caccagcagt ggtagcctca cccctgaaat
      301 ccagttgcgg cttttgaccc ccagatgcaa attctggtgg gagagagctg acctgtggcc
      361 ccacagtgat ccttactggg caatctactg gccaggaggc caagccctgt ctaggtatct
      421 tttggataat cctgatgttg tcagaggaaa atctgtatta gatcttggga gtggatgtgg
      481 agctacagct attgctgcta agatgagtgg ggcatcaagg atcttggcca atgacataga
      541 ccctattgca ggaatggcta ttacactaaa ttgtgaattg aacagactga atccttttcc
      601 tattttaatc caaaacattt tgaatttgga acaagataag tgggaccttg ttgttcttgg
      661 cgatatgttt tatgatgaag accttgcaga tagtcttcat cagtggctga agaagtgctt
      721 ctggacctat agaactcgag tactgattgg tgaccctggg cggccccagt tcagtggaca
      781 cagcattcag catcacctgc acaaagtggt agaatattca cttttggagt ctactaggca
      841 ggaaaacagt ggactgacaa caagcacagt gtggggtttt cagccttgag cggccgcact
      901 cgagcaccac caccaccac
//
