LOCUS       sequence-165985-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone-MarkerFree backbone vector for the addition of 1 or 2 
            genes and promoters for integration into site XI-2 (Chr XI: 
            91575..92913).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, 
            Keasling JD, Borodina I
  TITLE     EasyClone-MarkerFree: A vector toolkit for marker-less integration 
            of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
  JOURNAL   Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. 
            Epub 2016 Jun 23.
  PUBMED    27166612
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201600147"; journalName: "Biotechnol J"; date: 
            "2016-08"; volume: "11"; issue: "8"; pages: "1110-7"
COMMENT     Sequence Label: pCfB2903(XI-2 MarkerFree)
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    174..207
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc
       61 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      121 gggcctttcg cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc
      181 ctattctcta gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg
      241 gtttaatgag gattttcgat ggagcaggat gaggagaaat agtaccacat gtatatatcc
      301 attacaaaaa ggtttatata caattacaat agacccttgt tggggtttct gaaaaaagaa
      361 gtagtcgatg ccatcggcaa taatacggaa ttacgagaaa cacaatcccg atcctttttt
      421 gggtaattac ttcaccgatt ctaccgattt atcatgccaa aaaaaattca ccgtgggttc
      481 tagaagtgcc ctttgaggat tgtagccact ctaacccaca cggcctcctt actagctgac
      541 taaggtgaca aaaccgcaag gactggaaag tcgccactca tctgaaaatt ctcaagtttt
      601 tcactactga gtttatgctt tcgaattttt ttgttcggta atagcacggc ggttcgattc
      661 aattccgccg ctccgagcga tgctccgcaa aactcagtaa taagctttct gatggttcac
      721 ccctttttta gcacgcgggg tgtaactcaa cagaaaaatg tgccatagaa caagactagg
      781 caaaagccaa ggagcgtttg ccatgaactt ccacaaatta agtcctcagc gagctcgcat
      841 ggaatgcgtg cgatgagcga cctcatgcta tacctgagaa agcaacctga cctacaggaa
      901 agagttactc a
//
