LOCUS       sequence-165991-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of YJR129c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Davydova E, Ho AY, Malecki J, Moen A, Enserink JM, Jakobsson ME, 
            Loenarz C, Falnes PO
  TITLE     Identification and characterization of a novel evolutionarily 
            conserved lysine-specific methyltransferase targeting eukaryotic 
            translation elongation factor 2 (eEF2).
  JOURNAL   J Biol Chem. 2014 Oct 31;289(44):30499-510. doi: 
            10.1074/jbc.M114.601658. Epub 2014 Sep 17.
  PUBMED    25231979
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.601658"; journalName: "J Biol Chem"; date: 
            "2014-10-31- 31"; volume: "289"; issue: "44"; pages: "30499-510"
COMMENT     Sequence Label: pET28a-YJR129c
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggtgc cgcgcggcag ccatatgaat gaagatctat
      121 tctacgatag attacatcag cggtgccctg gaaaatactt gctagaagag ttggaaacaa
      181 gcaaatctaa cgatgtcctg catgcatcga gatttgtttg tgagatggaa cttgtgcaaa
      241 agacgaatgc atattattgc aaaaccatag tcaagatgct cttagaccac gaatggattt
      301 tcgcgaaggc attcacaatt gtcaacgatg gcgaagacga gatagagata tatgattatc
      361 tctatgagaa gtacataaaa ctattaagca caggcaagcc agacccgatg atgaaagacg
      421 tcgtgaggta caggtttgac gaggacgtaa agatcaaaat tgaggaaaca ccgaatttga
      481 tcagtgcggc cagtactaca ggtttcagga catgggaagc tgccttatac atgggtgatt
      541 tccttattca taagccctta caagagttgg caccggtaca aggacaagac gatggcaaga
      601 aaaaattaaa tgttcttgag gtcggcgctg gcacaggcat agtgagtctc gtcattttac
      661 aaaagtatca cgagttcgta aataagatgt acgtcacgga tggtgattcg aatctcgttg
      721 agacgcagct gaaaagaaat tttgaattga acaatgaagt gcgtgagaac gaaccagaca
      781 tcaagttgca aagattatgg tggggcagcg atagagttcc tgaggatata gatttagtag
      841 tgggggcaga tgttacgtat gacccaacta tccttcctga tctatgcgag tgtctggccg
      901 aatgtcttgc tttagaccga tgcaaactg
//
