LOCUS       sequence-166016-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Gli3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  TITLE     Fernandez-Zapico lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: hGli3 6x his
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             123..140
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             144..176
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(180..188,189..203)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttacc atggggggtt
      121 ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa atgggtcggg
      181 atctgtacga cgatgacgat aaggtaccag gatccagtgt ggtggaattc tgcagatatc
      241 cagcacagtg gcggccgctc gagtctagaa tggaggccca gtcccacagc tccacgacca
      301 ctgaaaagaa aaaagttgag aattccatag tgaagtgctc cactcgaaca gatgtgagcg
      361 agaaagccgt tgcctccagc accacttcta atgaggatga aagtcctgga cagacttatc
      421 acagagagag aagaaacgca atcactatgc agccacagaa tgtccagggg ctcagcaaag
      481 tcagtgagga accttcaaca tcgagtgacg agagggcctc attgatcaag aaagagatcc
      541 atgggtccct gccacacgtg gcggagccct ctgtgccgta ccgcgggacg gtgtttgcca
      601 tggaccccag gaatggttac atggagcccc actaccaccc tcctcatctt ttccctgcct
      661 tccatcctcc tgtaccaatt gatgccagac atcatgaggg ccgttaccat tacgatccat
      721 ctccgattcc tccattgcat atgacttccg ccttatctag tagccctacg tatccggacc
      781 tgcccttcat taggatctcc ccacaccgga accccactgc tgcttccgag tctcccttca
      841 gccctccaca tccctacatt aatccctaca tggactatat ccgctccttg cacagcagcc
      901 catcgctctc
//
