LOCUS       sequence-16613-e        1410 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD91_14
FEATURES             Location/Qualifiers
     source          1..1410
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             357..377
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(420..448)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gnngnggtcg actctagagg atccccaatt cttgagactt ccactatctt ctctggcacc
       61 ctccactacg cgtaggcgtc tctcgcttcg tgtattcccg ggaagccggg ttcccgtctc
      121 tcccgccgct gccgctgccg cacacagctt tacacctcgt agaatcccca aagaggggcg
      181 tggcttgcgg gtgccaacat cctcctgccg aggaagaagc aggcactcat cactcgcatc
      241 atcaacctcg ggattggcca aaggacccaa aggtatgttt cgaatgatac taacataaca
      301 tagaacattt tcaggaggac ccttggaggg taccgagctc agaaaaaatg actgctccaa
      361 agaagaagcg taaggtaccg gtagaaaaaa tgagtaaagg agaagaactt ttcactggag
      421 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
      481 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
      541 gaaaactacc tgttccatgg ccaacacttg tcactacttt ctcttatggt gttcaatgct
      601 tttcaagata cccagatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag
      661 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
      721 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
      781 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
      841 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcnaaatt agacacaaca
      901 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
      961 gncctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaangatc
     1021 ccaacgaaan agagaccaca tggtccttct ganttgtaac agctgctggg ataccntggc
     1081 tggatgaact atacaantag catcntaaan tncnactgag cgcnggcgct accatnacaa
     1141 cttgnnnggg ncaaaanaaa ngggcnntgn tatnaantnc cntcccngnc cgggccttga
     1201 atttaannca atnatcctna aatccnaang gcttttcccg gggncccccc ccattttntt
     1261 ttaanaaaac ttccntantt ttnttttnnc ctcttnancc ccccaaagna tngggaatcc
     1321 aaaaaatttn cnaaaannca aaaatggccc cccccnnaaa ttnccaancn ngggggggcc
     1381 tttttttttn tattttnttn nnaaanccnc
//
