LOCUS       sequence-166160-        7732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Onder TT, Gupta PB, Mani SA, Yang J, Lander ES, Weinberg RA
  TITLE     Loss of E-cadherin promotes metastasis via multiple downstream 
            transcriptional pathways.
  JOURNAL   Cancer Res. 2008 May 15. 68(10):3645-54.
  PUBMED    18483246
REFERENCE   2  (bases 1 to 7732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2008 May 15. 68(10):3645-54."
COMMENT     Sequence Label: pWZL Blast mouse E-cadherin
FEATURES             Location/Qualifiers
     source          1..7732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    4097..4558
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4153..4170)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4380..4399
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             5174..5643
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5825..5842)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5996..6584)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6076..6095)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6744..7535,7536..7604))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7367..7386
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7605..7709)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatctgc cgggtccgcc
     1381 atgggagccc ggtgccgcag cttttccgcg ctcctgctcc tgctgcaggt ctcctcatgg
     1441 ctttgccagg agctggagcc tgagtcctgc agtcccggct tcagttccga ggtctacacc
     1501 ttcccggtgc cggagaggca cctggagaga ggccatgtcc tgggcagagt gagatttgaa
     1561 ggatgcaccg gccggccaag gacagccttc ttttcggaag actcccgatt caaagtggcg
     1621 acagacggca ccatcacagt gaagcggcat ctaaagctcc acaagctgga gaccagtttc
     1681 ctcgtccgcg cccgggactc cagtcatagg gagctgtcta ccaaagtgac gctgaagtcc
     1741 atggggcacc accatcaccg gcaccaccac cgcgaccctg cctctgaatc caacccagag
     1801 ctgctcatgt ttcccagcgt gtacccaggt ctcagaagac agaaacgaga ctgggtcatc
     1861 cctcccatca gctgccccga aaatgaaaag ggtgaattcc caaagaacct ggttcagatc
     1921 aaatccaaca gggacaaaga aacaaaggtt ttctacagca tcaccggcca aggagctgac
     1981 aaaccccccg ttggcgtttt catcattgag agggagacag gctggctgaa agtgacacag
     2041 cctctggata gagaagccat tgccaagtac atcctctatt ctcatgccgt gtcatcaaat
     2101 ggggaagcgg tggaggatcc catggagata gtgatcacag tgacagatca gaatgacaac
     2161 aggccagagt ttacccagga ggtgtttgag ggatccgttg cagaaggcgc tgttccagga
     2221 acctccgtga tgaaggtctc agccaccgat gcagacgatg acgtcaacac ctacaacgct
     2281 gccatcgcct acaccatcgt cagccaggat cctgagctgc ctcacaaaaa catgttcact
     2341 gtcaataggg acaccggggt catcagtgtg ctcacctctg ggctggaccg agagagttac
     2401 cctacataca ctctggtggt tcaggctgct gaccttcaag gcgaaggctt gagcacaaca
     2461 gccaaggctg tgatcactgt caaggatatt aatgacaacg ctcctgtctt caacccgagc
     2521 acgtatcagg gtcaagtgcc tgagaatgag gtcaatgccc ggatcgccac actcaaagtg
     2581 accgatgatg atgcccccaa cactccggcg tggaaagctg tgtacaccgt agtcaacgat
     2641 cctgaccagc agttcgttgt cgtcacagac cccacgacca atgatggcat tttgaaaaca
     2701 gccaagggct tggattttga ggccaagcag caatacatcc ttcatgtgag agtggagaac
     2761 gaggaaccct ttgaggggtc tcttgtccct tccacagcca ctgtcactgt ggacgtggta
     2821 gacgtgaatg aagcccccat ctttatgcct gcggagagga gagtcgaagt gcccgaagac
     2881 tttggtgtgg gtcaggaaat cacatcttat accgctcgag agccggacac gttcatggat
     2941 cagaagatca cgtatcggat ttggagggac actgccaact ggctggagat taacccagag
     3001 actggtgcca ttttcacgcg cgctgagatg gacagagaag acgctgagca tgtgaagaac
     3061 agcacatatg tagctctcat catcgccaca gatgatggtt cacccattgc cactggcacg
     3121 ggcactcttc tcctggtcct gttagacgtc aatgacaacg ctcccatccc agaacctcga
     3181 aacatgcagt tctgccagag gaacccacag cctcatatca tcaccatctt ggatccagac
     3241 cttcccccca acacgtcccc ctttactgct gagctaaccc atggggccag cgtcaactgg
     3301 accattgagt ataatgacgc agctcaagaa tctctcattt tgcaaccaag aaaggactta
     3361 gagattggcg aatacaaaat ccatctcaag ctcgcggata accagaacaa agaccaggtg
     3421 accacgttgg acgtccatgt gtgtgactgt gaagggacgg tcaacaactg catgaaggcg
     3481 ggaatcgtgg cagcaggatt gcaagttcct gccatcctcg gaatccttgg agggatcctc
     3541 gccctgctga ttctgatcct gctgctccta ctgtttctac ggaggagaac ggtggtcaaa
     3601 gagcccctgc tgccaccaga tgatgatacc cgggacaatg tgtattacta tgatgaagaa
     3661 ggaggtggag aagaagacca ggactttgat ttgagccagc tgcacagggg cctggatgcc
     3721 cgaccggaag tgactcgaaa tgatgtggct cccaccctca tgagcgtgcc ccagtatcgt
     3781 ccccgtcctg ccaatcctga tgaaattgga aacttcatcg atgaaaacct gaaggcagcc
     3841 gacagcgacc ccacggcacc cccttacgac tctctgttgg tgttcgatta cgagggcagt
     3901 ggttctgaag ccgctagcct gagctcactg aactcctctg agtcggatca ggaccaggac
     3961 tacgattatc tgaacgagtg gggcaaccga ttcaagaagc tggcggacat gtacggcggt
     4021 ggcgaagacg actaggggac tagcaagtcg accaattccg gttattttcc accatattgc
     4081 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     4141 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     4201 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     4261 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     4321 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     4381 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     4441 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     4501 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     4561 ccatgaaaac atttaacatt tctcaacagg atctagaatt agtagaagta gcgacagaga
     4621 agattacaat gctttatgag gataataaac atcatgtggg agcggcaatt cgtacgaaaa
     4681 caggagaaat catttcggca gtacatattg aagcgtatat aggacgagta actgtttgtg
     4741 cagaagccat tgcgattggt agtgcagttt cgaatggaca aaaggatttt gacacgattg
     4801 tagctgttag acacccttat tctgacgaag tagatagaag tattcgagtg gtaagtcctt
     4861 gtggtatgtg tagggagttg atttcagact atgcaccaga ttgttttgtg ttaatagaaa
     4921 tgaatggcaa gttagtcaaa actacgattg aagaactcat tccactcaaa tatacccgaa
     4981 attaaaagtt ttaccatacc aagctcatcg ataaaataaa agattttatt tagtctccag
     5041 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca
     5101 ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc aaggtcagga
     5161 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     5221 ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     5281 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct
     5341 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc
     5401 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct
     5461 ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc tccgattgac
     5521 tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg
     5581 tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt
     5641 tcacatgcag catgtatcaa aattaatttg gttttttttc ttaagtattt acattaaatg
     5701 gccatagttg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5761 ctcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5821 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5881 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5941 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6001 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6061 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6121 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6181 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6241 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6301 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6361 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6421 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6481 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6541 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6601 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6661 gattatcaaa aaggatcttc acctagatcc ttttgcggcc gcaaatcaat ctaaagtata
     6721 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6781 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6841 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6901 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6961 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7021 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7081 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7141 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7201 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7261 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7321 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7381 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7441 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7501 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7561 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7621 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7681 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//
