LOCUS       sequence-166166-        3646 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Msx2. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Msx2
FEATURES             Location/Qualifiers
     source          1..3646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(771..789)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(772..789)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(807..823)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(807..823)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(820..842)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    831..847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(855..861,862..879,880..885))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    900..921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1038..1055)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1209..1797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1289..1308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1968..2759,2760..2828))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2591..2610
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2829..2933)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3011..3466)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3148..3169)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3360..3379
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3592..3614
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3606..3623
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3607..3623
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3630..3646,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3630..3646,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tttgacctgg gtctctgtaa ggttcagaga gctggagaac tcggcccgct ctgctatgga
      121 caggtactgt ttctggcgga acttgcgctc caaggctaga agctgggatg tggtgaaggg
      181 tgtgcgtggc ttccggttgg tcttgtgttt cctcagggtg caggtggtgg ggctcatatg
      241 tctgggcggc ggggagtatc tgccgggctc ctgtatccac ggtgctccgt cttcggaatt
      301 ttccgacttg accgaggcgg tctcgaaggg cttgacgaga ggcccgggac tgtgagcgtc
      361 ccggacgccg tgtcccggca gcagcagcgg ccgcaggaca gcgccagccg aggcgcagtc
      421 gggtggcacc gcgggcgatt ccttgggcgg cttcttgtcg gacatgagcg cctccacgct
      481 gaagggcagg ctggagacct tgaccctgcg ttcctctgcg ctgccctcgg ctcctccagg
      541 cccgggaccc gggccggcca gtaccgcggg gccctcctca tccgacgaaa acaagtcacc
      601 gcctttagtc ggagaagcca tgactttctg cgccgcgtag ctgtcggcgc gcgactccgc
      661 tggcaactct caagcgaaga ctccaacttt aatcactagt gaattcgcgg ccgcctgcag
      721 gtcgaccata tgggagagct cccaacgcgt tggatgcata gcttgagtat tctatagtgt
      781 cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      841 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      901 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      961 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1021 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1081 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1141 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1201 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1261 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1321 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1381 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1441 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1501 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1561 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1621 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1681 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1741 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1801 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1861 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1921 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1981 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2041 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2101 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2161 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2221 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2281 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2341 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2401 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2461 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2521 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2581 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2641 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2701 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2761 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2821 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2881 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2941 ccccgaaaag tgccacctga tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     3001 ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     3061 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     3121 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     3181 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     3241 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     3301 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     3361 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     3421 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca
     3481 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     3541 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     3601 gacgttgtaa aacgacggcc agtgaattgt aatacgactc actata
//
