LOCUS       sequence-166174-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Pbx1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Pbx1
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(407..425)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(408..425)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(443..459)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(443..459)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(456..478)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    467..483
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(491..497,498..515,516..521))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    536..557
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(674..691)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcccggccg ccatggcggc cgcgggaatt cgattgactt ctctcactgg caggtctggg
       61 ttagtaacaa agcatagact atggagttag ttaggtacat ctaagcctga aaataggctc
      121 taggtttttt ttatctatgt ggccatgggc aagttactaa atatttcgga ctctcaattt
      181 tcattattta ttagaggata ggaattcctc ttggagatgt ggtagggatt agtcaaaata
      241 atacatgtgc aacccctccc acagtgactg acatgtggga gatgctatac ctgcaaccat
      301 gttctctccc ctatgcgatt agttgtaatc actagtgaat tcgcggccgc ctgcaggtcg
      361 accatatggg agagctccca acgcgttgga tgcatagctt gagtattcta tagtgtcacc
      421 taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      481 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      541 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
      601 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
      661 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      721 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      781 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaag
//
