LOCUS       sequence-166236-         604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of shRNAs, compatible with double-shRNA 
            expression, EF1alpha promoter, mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Kampmann M, Bassik MC, Weissman JS
  TITLE     Integrated platform for genome-wide screening and construction of 
            high-density genetic interaction maps in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jun 18;110(25):E2317-26. doi: 
            10.1073/pnas.1307002110. Epub 2013 Jun 5.
  PUBMED    23739767
REFERENCE   2  (bases 1 to 604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1307002110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-06-18- 18"; volume: "110"; issue: "25"; pages: 
            "E2317-26"
COMMENT     Sequence Label: pMK1200
FEATURES             Location/Qualifiers
     source          1..604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(213..227)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattgta cctagtggaa
       61 ccggaaccct taaacatgtc tcgacctggc ttactagtcg gttcaactct agatatgtgg
      121 tatagatcgg aagagcgtcg tgtagggaaa gagtgtgact gaccccttga agcccgaggc
      181 ccaaggcagt gggcaggtcc tgaagttcat tacatctgtg gcttcactaa tgaacttcag
      241 acctgcccaa cgctctgggt caacagcaat ataccttctt ctcgagtcaa ggcgaatacc
      301 taggcaattc ttacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc
      361 gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt
      421 tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtangtg gtcttgacct
      481 cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg
      541 cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca
      601 ttac
//
