LOCUS       sequence-166310-        6092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Xenopus laevis oocyte expression of Kv10.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Jenke M, Sanchez A, Monje F, Stuhmer W, Weseloh RM, Pardo LA
  TITLE     C-terminal domains implicated in the functional surface expression 
            of potassium channels.
  JOURNAL   EMBO J. 2003 Feb 3;22(3):395-403.
  PUBMED    12554641
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2003-02-3"; volume: "22(3)"; pages: "395-403"
COMMENT     Sequence Label: pSGEMKv10.1
FEATURES             Location/Qualifiers
     source          1..6092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           34..76
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             complement(1817..1831)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     primer_bind     complement(3133..3149)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3134..3150)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     3'UTR           3183..3308
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     promoter        complement(3417..3435)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3418..3435)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3453..3469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3453..3469)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3466..3488)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3477..3493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3501..3507,3508..3525,3526..3531))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3546..3567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3684..3701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3855..4443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3935..3954)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4614..5405,5406..5474))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5237..5256
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5475..5579)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5647..5665
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5703..5725)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5825..5844
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6038..6060
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6052..6069
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6053..6069
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(6076..6092,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(6076..6092,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaatta attcgagctc ggtacccagc ttgcttgttc tttttgcaga agctcagaat
       61 aaacgctcaa ctttggcaga tcaattcccc caccgcggtg gcggccggaa ttccgggccc
      121 gccggacccc gagctgctgg gaggatgacc atggctgggg gcaggagggg actagtggcc
      181 cctcaaaaca cgtttctgga gaatattgtt cggcggtcca atgatactaa ttttgtgttg
      241 gggaatgctc agatagtgga ctggcctatt gtgtacagca atgatggatt ttgcaagctg
      301 tctggctatc acagggcaga agtgatgcaa aaaagcagca cctgcagttt tatgtatggg
      361 gagctgactg ataaagacac gattgaaaaa gtgcggcaaa catttgagaa ctatgagatg
      421 aattcctttg aaattctgat gtacaagaag aacaggacac ctgtgtggtt ctttgtgaaa
      481 attgctccaa ttcgaaacga acaggataaa gtggttttat ttctttgcac tttcagtgac
      541 ataacagctt tcaaacagcc aattgaggat gattcatgta aaggctgggg gaagtttgct
      601 cggctgacaa gagcactgac aagcagcagg ggtgtcctgc agcagctggc tccaagcgtg
      661 caaaaaggcg agaatgtcca caagcactcc cgcctggcag aggtcctaca gctgggctca
      721 gacatccttc cccagtacaa gcaagaggca ccaaagactc cccctcacat catcttacat
      781 tattgtgttt ttaagaccac gtgggattgg atcatcttga tcttgacctt ctatacagcc
      841 atcttggtcc cttataatgt ctccttcaaa accaggcaga ataatgtggc ctggctggtt
      901 gttgatagca tcgtggatgt tatctttttg gtggacattg tgctcaattt tcataccacc
      961 tttgttggac cagcagggga ggtgatttct gaccccaaac ttatccgcat gaactacctg
     1021 aagacgtggt ttgtgattga ccttctgtcc tgtttgccat atgatgtcat caacgctttt
     1081 gagaacgtgg atgagggcat cagcagcctg ttcagctctc taaaagttgt ccggctgctc
     1141 cgtcttgggc gagtggcccg taagctggac cactacattg aatatggagc tgctgtgctg
     1201 gtcctgctgg tgtgtgtgtt tgggctggct gcacactgga tggcctgcat ctggtacagc
     1261 attggggact atgagatctt tgacgaggac accaagacaa tccgcaacaa cagctggctg
     1321 taccaactag cgatggacat tggcacccct taccagttta atgggtctgg ctcagggaag
     1381 tgggaaggtg gtcccagcaa gaattctgtc tacatctcct cgttgtattt cacaatgacc
     1441 agcctcacca gtgtgggctt tgggaacatc gccccatcca cagacattga gaagatcttt
     1501 gcagtggcca tcatgatgat tggctcactt ctctatgcca ccatcttcgg gaatgtgacg
     1561 actattttcc aacagatgta tgccaacacc aacagatacc atgagatgct caacagtgtt
     1621 cgggacttcc tgaagctcta ccaggtgcca aaaggattga gtgagcgagt aatggattat
     1681 attgtgtcca cttggtccat gtccagaggc attgacacag agaaggtcct gcagatctgc
     1741 cccaaggaca tgagagccga catctgcgtg cacctgaacc gcaaggtgtt caaggagcac
     1801 ccggccttcc ggctggccag tgatggctgc ctccgggcac tggccatgga gttccagacg
     1861 gtgcactgtg ccccagggga cctcatctac catgcaggag agagcgttga cagcctctgc
     1921 tttgtggttt ctggctccct ggaggtgatc caagatgatg aggtggtggc cattctagga
     1981 aaaggagacg tgtttggaga tgtgttctgg aaggaagcca cccttgccca gtcctgtgcc
     2041 aatgttaggg ccttgaccta ctgtgatctg catgtgatca agcgggatgc cctgcagaaa
     2101 gtgctggaat tctacacggc cttctcccat tccttctccc ggaacctgat tctgacgtac
     2161 aacttgagga agaggattgt gttccggaag atcagcgatg tgaaacgtga agaggaagaa
     2221 cgcatgaaac gaaagaatga ggcccccctg atcttgcccc cggaccaccc tgtccggcgc
     2281 ctcttccaga gattccgaca gcagaaagag gccaggctgg cagctgagag agggggccgg
     2341 gacctggatg acctagatgt ggagaagggc aatgtcctta cagagcatgc ctccgccaac
     2401 cacagcctcg tgaaggccag cgtggtcacc gtgcgtgaga gtcctgccac gcccgtatcc
     2461 ttccaggcag cctccacctc cggggtgcca gaccacgcaa agctacaggc gccagggtcc
     2521 gagtgcctgg gccccaaggg gggcgggggc gattgtgcca agcgcaaaag ctgggcccgc
     2581 ttcaaagatg cttgcgggaa gagtgaggac tggaacaagg tgtccaaggc tgagtcgatg
     2641 gagacacttc ccgagaggac aaaagcgtca ggcgaggcca cactgaagaa gacagactcg
     2701 tgtgacagtg gcatcaccaa gagcgacttg cgcctggaca acgtgggtga ggccaggagt
     2761 ccccaggatc ggagtcccat cctggcagag gtcaagcatt cgttctaccc catccctgag
     2821 cagacgctgc aggccacagt cctggaggtg aggcacgagc tgaaggagga catcaaggcc
     2881 ttaaacgcca aaatgaccaa tattgagaaa cagctctctg agatactcag gatattaact
     2941 tccagaagat cctctcagtc tcctcaggag ttgtttgaaa tatcgaggcc acagtcccca
     3001 gaatcagaga gagacatttt tggagccagc tgagaggtct atttaaaaaa aaagtcagag
     3061 acagatacct ccaaccctgc cgtcaccacc acccctacca cccgggctgc aggaattcga
     3121 tatcaagctt atcgataccg tcgacctcga gggggggccc gagcttgatc tggttaccac
     3181 taaaccagcc tcaagaacac ccgaatggag tctctaagct acataatacc aacttacact
     3241 ttacaaaatg ttgtccccca aaatgtagcc attcgtatct gctcctaata aaaagaaagt
     3301 ttcttcacat tctaaaaaaa aaaaaaaaaa aaaaaacccc cccccccccc cccccccccc
     3361 ccccccctgc aggcatgcat taattaaagg ccatgatggc cagctagctt gagtattcta
     3421 tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3481 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3541 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3601 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3661 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3721 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3781 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3841 gcgttgctgg cgtttttcga taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3901 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3961 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4021 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4081 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4141 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4201 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4261 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     4321 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4381 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4441 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4501 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4561 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4621 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4681 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4741 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4801 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4861 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4921 ggcattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4981 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5041 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5101 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5161 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5221 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5281 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5341 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5401 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5461 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5521 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5581 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     5641 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     5701 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     5761 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     5821 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     5881 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     5941 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     6001 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     6061 acggccagtg aattgtaata cgactcacta ta
//
