LOCUS       sequence-166316-        9221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Wang L, Jin Q, Lee JE, Su IH, Ge K
  TITLE     Histone H3K27 methyltransferase Ezh2 represses Wnt genes to 
            facilitate adipogenesis.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Apr 20. 107(16):7317-22.
  PUBMED    20368440
REFERENCE   2  (bases 1 to 9221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Apr 20. 107(16):7317-22."
COMMENT     Sequence Label: MSCVhygro-F-Ezh2
FEATURES             Location/Qualifiers
     source          1..9221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..417
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     347..369
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     385..401
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      432..441
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             441..464
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        2728..3227
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2763..2786)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3160..3179
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3245..4270
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3245..4270
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             4628..5142
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5304..5320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5328..5334,5335..5352,5353..5358))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5373..5394
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5511..5528)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5682..6270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5762..5781)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6441..7232,7233..7301))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7064..7083
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7302..7406)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7474..7492
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7530..7552)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7652..7671
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7865..7887
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8015..8034
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             8234..8750
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    8814..9155
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
ORIGIN
        1 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
       61 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
      121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
      181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
      241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
      301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
      361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccgga
      421 attagatcct ggccaccatg gactacaagg acgacgatga caagatgggc cagactggga
      481 agaaatctga gaagggaccg gtttgttggc ggaagcgtgt aaaatcagag tacatgagac
      541 tgagacagct caagaggttc agaagagctg atgaagtaaa gactatgttt agttccaatc
      601 gtcagaaaat tttggaaaga actgaaacct taaaccaaga gtggaagcag cggaggatac
      661 agcctgtgca catcatgact tctgtgagct cattgcgcgg gactagggag tgttcagtca
      721 ccagtgactt ggattttcca gcacaagtca tcccgttaaa gaccctgaat gcagtcgcct
      781 cggtgcctat aatgtactct tggtcgccct tacaacagaa ttttatggtg gaagacgaaa
      841 ctgtcttaca taacattcct tacatggggg atgaagttct ggatcaggac ggcactttca
      901 ttgaagaact aataaaaaat tatgatggaa aagtgcatgg tgacagagaa tgtggattta
      961 taaatgatga aatttttgtg gagttggtaa atgctcttgg tcaatataat gatgatgatg
     1021 atgacgatga tggagatgat ccagatgaaa gagaagaaaa acagaaagat ctagaggata
     1081 atcgagatga taaagaaact tgcccacctc ggaaatttcc tgctgataaa atatttgaag
     1141 ccatttcctc aatgtttcca gataagggca ccgcagaaga actgaaggaa aaatataaag
     1201 aactcacgga gcagcagctc ccaggtgctc tgcctcctga atgtactcca aacatcgatg
     1261 gaccaaatgc caaatctgtt cagagggagc aaagcttgca ttcatttcat acgctcttct
     1321 gtcgacgatg ttttaagtat gactgcttcc tacatccctt ccatgcaaca cccaacacat
     1381 ataagaggaa gaacacagaa acagctttgg acaacaagcc ttgtggacca cagtgttacc
     1441 agcatctgga gggagctaag gagtttgctg ctgctcttac tgctgagcgg ataaagacac
     1501 cacctaaacg cccagggggc cgcagaagag gaagacttcc gaataacagt agcagaccca
     1561 gcacccccac catcagtgtg ctggagtcaa aggatacaga cagtgacaga gaagcaggga
     1621 ctgaaactgg gggagagaac aacgataaag aagaagaaga gaaaaaagat gagacgtcca
     1681 gctcctctga agcaaattct cggtgtcaaa caccaataaa gatgaagcca aatattgaac
     1741 ctcctgagaa tgtggagtgg agtggtgctg aagcctccat gtttagagtc ctcattggta
     1801 cttactacga taacttttgt gccattgcta ggctaattgg gaccaaaaca tgtagacagg
     1861 tgtatgagtt tagagtcaag gagtccagta tcatagcacc tgttcccact gaggatgtag
     1921 acactcctcc aagaaagaag aaaaggaaac atcggttgtg ggctgcacac tgcagaaaga
     1981 tacaactgaa aaaggacggc tcctctaacc atgtttacaa ctatcaaccc tgtgaccatc
     2041 cacggcagcc ttgtgacagt tcgtgccctt gtgtgatagc acaaaatttt tgtgaaaagt
     2101 tttgtcaatg tagttcagag tgtcaaaacc gctttcctgg atgtcggtgc aaagcacaat
     2161 gcaacaccaa acagtgtcca tgctacctgg ctgtccgaga gtgtgaccct gacctctgtc
     2221 tcacgtgtgg agctgctgac cattgggaca gtaaaaatgt atcctgtaag aactgtagca
     2281 ttcagcgggg ctctaaaaag cacttactgc tggcaccgtc tgatgtggca ggctggggca
     2341 tctttatcaa agatcctgta caaaaaaatg aattcatctc agaatactgt ggggagatta
     2401 tttctcagga tgaagcagac agaagaggaa aagtgtatga caaatacatg tgcagctttc
     2461 tgttcaactt gaacaatgat tttgtggtgg atgcaacccg aaagggcaac aaaattcgtt
     2521 ttgctaatca ttcagtaaat ccaaactgct atgcaaaagt tatgatggtt aatggtgacc
     2581 acaggatagg catctttgct aagagggcta tccagactgg tgaagagttg ttttttgatt
     2641 acagatacag ccaggctgat gccctgaagt atgtgggcat cgaacgagaa atggaaatcc
     2701 cttgactcga ggttaacgaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     2761 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     2821 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     2881 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     2941 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     3001 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     3061 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     3121 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     3181 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcatcccgc
     3241 caccatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     3301 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     3361 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     3421 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     3481 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     3541 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     3601 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     3661 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     3721 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     3781 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     3841 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     3901 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     3961 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     4021 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     4081 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     4141 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     4201 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     4261 aaaggaatag agtagatgcc gaccgaacaa gagctgattt cgagaacgcc tcagccagca
     4321 actcgcgcga gcctagcaag gcaaatgcga gagaacggcc ttacgcttgg tggcacagtt
     4381 ctcgtccaca gttcgctaag ctcgctcggc tgggtcgcgg gagggccggt cgcagtgatt
     4441 caggcccttc tggattgtgt tggtccccag ggcacgattg tcatgcccac gcactcgggt
     4501 gatctgactg atcccgcaga ttggagatcg ccgcccgtgc ctgccgattg ggtgcagatc
     4561 cgtcgacctg cagccaagct tatcgataaa ataaaagatt ttatttagtc tccagaaaaa
     4621 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
     4681 caaggcatgg aaaatacata actgagaata gagaagttca gatcaaggtt aggaacagag
     4741 agacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     4801 ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta gagaaccatc
     4861 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
     4921 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
     4981 cacaacccct cactcggcgc gccagtcctc cgatagactg cgtcgcccgg gtacccgtgt
     5041 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
     5101 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atgggtaaca gtttcttgaa
     5161 gttggagaac aacattctga gggtaggagt cgaatattaa gtaatcctga ctcaattagc
     5221 cactgttttg aatccacata ctccaatact cctgaaatag ttcattatgg acagcgcaga
     5281 aagagctggg gagaattgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     5341 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     5401 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5461 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5521 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5581 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5641 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5701 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5761 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5821 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5881 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5941 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6001 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6061 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6121 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6181 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6241 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6301 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6361 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6421 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6481 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6541 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6601 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6661 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6721 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6781 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6841 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6901 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6961 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7021 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7081 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7141 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7201 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7261 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7321 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7381 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     7441 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     7501 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     7561 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     7621 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     7681 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     7741 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     7801 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     7861 ttttcccagt cacgacgttg taaaacgacg gcgcaaggaa tggtgcatgc aaggagatgg
     7921 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     7981 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     8041 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggcgattag
     8101 tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc
     8161 accagctgaa gcctatagag tacgagccat agataaaata aaagatttta tttagtctcc
     8221 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     8281 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
     8341 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     8401 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag
     8461 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     8521 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     8581 aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta
     8641 cccgtattcc caataaagcc tcttgctgtt tgcatccgaa tcgtggactc gctgatcctt
     8701 gggagggtct cctcagattg attgactgcc cacctcgggg gtctttcatt tggaggttcc
     8761 accgagattt ggagacccct gcccagggac caccgacccc cccgccggga ggtaagctgg
     8821 ccagcggtcg tttcgtgtct gtctctgtct ttgtgcgtgt ttgtgccggc atctaatgtt
     8881 tgcgcctgcg tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
     8941 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
     9001 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
     9061 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
     9121 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
     9181 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta g
//
