LOCUS       sequence-166317-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Sanchez-Rivera FJ, Papagiannakopoulos T, Romero R, Tammela T, Bauer 
            MR, Bhutkar A, Joshi NS, Subbaraj L, Bronson RT, Xue W, Jacks T
  TITLE     Rapid modelling of cooperating genetic events in cancer through 
            somatic genome editing.
  JOURNAL   Nature. 2014 Oct 22. doi: 10.1038/nature13906.
  PUBMED    25337879
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Oct 22. doi: 10.1038/nature13906."
COMMENT     Sequence Label: pSECC
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(312..332)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(339..362)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      362..371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(416..627)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    726..843
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(891..966)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aagctgtcgt ccaccttggc catctcgttg ctgaagatct cttgcagata gcagatccgg
       61 ttcttccgtc tggtgtatct tcttctggcg gttctcttca gccgggtggc ctcggctgtt
      121 tcgccgctgt cgaacagcag ggctccgatc aggttcttct tgatgctgtg ccggtcggtg
      181 ttgcccagca ccttgaattt cttgctgggc accttgtact cgtcggtgat cacggcccag
      241 cccacagagt tggtgccgat gtccaggccg atgctgtact tcttgtcggc tgctgggact
      301 ccgtggatac cgaccttccg cttcttcttt ggggccatct tatcgtcatc gtctttgtaa
      361 tccatggtgg cccgcttaag ctgtagcgct aatgtcgggt cccgcgtcac gacacctgtg
      421 ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc gactactgca cttatatacg
      481 gttctccccc accctcggga aaaaggcgga gccagtacac gacatcactt tcccagttta
      541 ccccgcgcca ccttctctag gcaccggttc aattgccgac ccctcccccc aacttctcgg
      601 ggactgtggg cgatgtgcgc tctgcccact gacgggcacc ggagccactc gaaggcctcg
      661 ggattcctag gaacagcggt ttgccggcgc caaagtggat ctctgctgtc cctgtaataa
      721 acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt
      781 gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta
      841 aaattgtgga tgaatactgc catttgtctc aagatctaga attcaaaaaa gcaccgactc
      901 ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta tttctagctc
      961 taaaacagag acgtac
//
