LOCUS       sequence-166318-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Sanchez-Rivera FJ, Papagiannakopoulos T, Romero R, Tammela T, Bauer 
            MR, Bhutkar A, Joshi NS, Subbaraj L, Bronson RT, Xue W, Jacks T
  TITLE     Rapid modelling of cooperating genetic events in cancer through 
            somatic genome editing.
  JOURNAL   Nature. 2014 Oct 22. doi: 10.1038/nature13906.
  PUBMED    25337879
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Oct 22. doi: 10.1038/nature13906."
COMMENT     Sequence Label: pSECC
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(102..122)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(126..182)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(198..245)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tggcgatccc tgaacatgtc catcaggttc ttgcgaacct catcactcgt tgcatcgacc
       61 ggtaatgcag gcaaattttg gtgtacggtc agtaaattgg acaccttcct cttcttcttg
      121 ggcatggggc cggggttctc ctccacgtcg ccggcctgct tcagcaggct gaagttggtg
      181 gcgccgctgc cgctagcttt ctttttctta gcttgaccag ctttcttagt agcagcagga
      241 cgcttgtcgc ctcccagctg agacaggtcg atccgtgtct cgtacaggcc ggtgatgctc
      301 tggtggatca gggtggcgtc cagcacctct ttggtgctgg tgtacctctt ccggtcgatg
      361 gtggtgtcaa agtacttgaa ggcggcaggg gctcccagat tggtcagggt aaacaggtgg
      421 atgatattct cggcctgctc tctgatgggc ttatcccggt gcttgttgta ggcggacagc
      481 actttgtcca gattagcgtc ggccaggatc actctcttgg agaactcgct gatctgctcg
      541 atgatctcgt ccaggtagtg cttgtgctgt tccacaaaca gctgtttctg ctcattatcc
      601 tcgggggagc ccttcagctt ctcatagtgg ctggccaggt acaggaagtt cacatatttg
      661 gagggcaggg ccagttcgtt tcccttctgc agttcgccgg cagaggccag cattctcttc
      721 ctgccgtttt ccagctcgaa cagggagtac ttaggcagct tgatgatcag gtcctttttc
      781 acttctttgt agcccttggc ttccagaaag tcgatgggat tcttctcgaa gctgcttctt
      841 tccatgatgg tgatccccag cagctctttc acactcttca gtttcttgga cttgcccttt
      901 tccactttgg ccaccaccag cacagaatag gccacggtgg ggctgtcgaa gccgc
//
