LOCUS       sequence-166319-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven
            Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) 
            Endogenous TAP Tagging..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, 
            Doyon Y
  TITLE     A Scalable Genome-Editing-Based Approach for Mapping Multiprotein 
            Complexes in Human Cells.
  JOURNAL   Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009.
  PUBMED    26456817
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009."
COMMENT     Sequence Label: AAVS1_Puro_PGK1_3xFLAG_Twin_Strep
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(56..79,80..115,116..139,140..145))
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             complement(155..220)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(239..259)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     promoter        complement(288..798)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(351..369)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ctggcaacta gaaggcacag cctgcagggt ttaaacgtct agactcgagt tatcactttt
       61 cgaactgggg gtggctccaa gcagatcctc cgcttccgcc gccagagcct ccgcccttct
      121 caaactgagg gtgggaccag gcgctgctgg cgcccttgtc atcgtcgtct ttgtagtcga
      181 tgtcgtgatc cttataatcg ccgtcgtggt ccttgtagtc gccggcggat cccatggctc
      241 cctgaaaata cagattctcg gcggccgcct taaggctgag ggtacccctg gggagagagg
      301 tcggtgattc ggtcaacgag ggagccgact gccgacgtgc gctccggagg cttgcagaat
      361 gcggaacacc gcgcgggcag gaacagggcc cacactaccg ccccacaccc cgcctcccgc
      421 accgcccctt cccggccgct gctctcggcg cgccctgctg agcagccgct attggccaca
      481 gcccatcgcg gtcggcgcgc tgccattgct ccctggcgct gtccgtctgc gagggtacta
      541 gtgagacgtg cggcttccgt ttgtcacgtc cggcacgccg cgaaccgcaa ggaaccttcc
      601 cgacttaggg gcggagcagg aagcgtcgcc ggggggccca caagggtagc ggcgaagatc
      661 cgggtgacgc tgcgaacgga cgtgaagaat gtgcgagacc cagggtcggc gccgctgcgt
      721 ttcccggaac cacgcccaga gcagccgcgt ccctgcgcaa acccagggct gccttggaaa
      781 aggcgcaacc ccaaccccgt ggatgcatta aa
//
