LOCUS       sequence-166322-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of MK03. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: MK03_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..83
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             103..132
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             133..153
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggctccggag agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat
       61 tttgtttaac tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat
      121 caccatcacc atgaaaacct gtacttccaa tccaatgcaa tggcggcggc ggcggctcag
      181 gggggcgggg gcggggagcc ccgtagaacc gagggggtcg gcccgggggt cccgggggag
      241 gtggagatgg tgaaggggca gccgttcgac gtgggcccgc gctacacgca gttgcagtac
      301 atcggcgagg gcgcgtacgg catggtcagc tcggcctatg accacgtgcg caagactcgc
      361 gtggccatca agaagatcag ccccttcgaa catcagacct actgccagcg cacgctccgg
      421 gagatccaga tcctgctgcg cttccgccat gagaatgtca tcggcatccg agacattctg
      481 cgggcgtcca ccctggaagc catgagagat gtctacattg tgcaggacct gatggagact
      541 gacctgtaca agttgctgaa aagccagcag ctgagcaatg accatatctg ctacttcctc
      601 taccagatcc tgcggggcct caagtacatc cactccgcca acgtgctcca ccgagatcta
      661 aagccctcca acctgctcat caacaccacc tgcgacctta agatttgtga tttcggcctg
      721 gcccggattg ccgatcctga gcatgaccac accggcttcc tgacggagta tgtggctacg
      781 cgctggtacc gggccccaga gatcatgctg aactccaagg gctataccaa gtccatcgac
      841 atctggtctg tgggctgcat tctggctgag atgctctcta accggcccat cttccctggc
      901 aagcactacc tggatcagct ca
//
