LOCUS       sequence-166339-        3918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SPOP, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SPOP_p.K101I
FEATURES             Location/Qualifiers
     source          1..3918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      28..616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     517..536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     770..787
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(946..973)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1065..1151)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1200..1222
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1214..1231
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1215..1231
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1247..1346
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2475..2574)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2591..2610)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2592..2610)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2615..2631)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2615..2631)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3062..3853
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
       61 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      121 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      181 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      241 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      301 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      361 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      421 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      481 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      541 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      601 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      661 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      721 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      781 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      841 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      901 aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc
      961 aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac
     1021 cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg
     1081 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt
     1141 cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc
     1201 ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccccaaa taatgatttt
     1261 attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat
     1321 gccaactttg tacaaaaaag ttggcaccat gtcaagggtt ccaagtcctc cacctccggc
     1381 agaaatgtcg agtggccccg tagctgagag ttggtgctac acacagatca aggtagtgaa
     1441 attctcctac atgtggacca tcaataactt tagcttttgc cgggaggaaa tgggtgaagt
     1501 cattaaaagt tctacatttt catcaggagc aaatgataaa ctgaaatggt gtttgcgagt
     1561 aaaccccaaa gggttagatg aagaaagcaa agattacctg tcactttacc tgttactggt
     1621 cagctgtcca aagagtgaag ttcgggcaat attcaaattc tccatcctga atgccaaggg
     1681 agaagaaacc aaagctatgg agagtcaacg ggcatatagg tttgtgcaag gcaaagactg
     1741 gggattcaag aaattcatcc gtagagattt tcttttggat gaggccaacg ggcttctccc
     1801 tgatgacaag cttaccctct tctgcgaggt gagtgttgtg caagattctg tcaacatttc
     1861 tggccagaat accatgaaca tggtaaaggt tcctgagtgc cggctggcag atgagttagg
     1921 aggactgtgg gagaattccc ggttcacaga ctgctgcttg tgtgttgccg gccaggaatt
     1981 ccaggctcac aaggctatct tagcagctcg ttctccggtt tttagtgcca tgtttgaaca
     2041 tgaaatggag gagagcaaaa agaatcgagt tgaaatcaat gatgtggagc ctgaagtttt
     2101 taaggaaatg atgtgcttca tttacacggg gaaggctcca aacctcgaca aaatggctga
     2161 tgatttgctg gcagctgctg acaagtatgc cctggagcgc ttaaaggtca tgtgtgagga
     2221 tgccctctgc agtaacctgt ccgtggagaa cgctgcagaa attctcatcc tggccgacct
     2281 ccacagtgca gatcagttga aaactcaggc agtggatttc atcaactatc atgcttcgga
     2341 tgtcttggag acctctgggt ggaagtcaat ggtggtgtca catccccact tggtggctga
     2401 ggcataccgc tctctggctt cagcacagtg cccttttctg ggacccccac gcaaacgcct
     2461 gaagcaatcc taattgccaa ctttcttgta caaagttggc attataaaaa agcattgctt
     2521 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     2581 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     2641 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct
     2701 cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg
     2761 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg
     2821 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     2881 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     2941 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     3001 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     3061 tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     3121 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     3181 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     3241 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     3301 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     3361 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     3421 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     3481 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     3541 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     3601 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     3661 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     3721 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     3781 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     3841 agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga gttacgcgtc
     3901 gttccactga gcgtcaga
//
