LOCUS       sequence-166344-        3918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SPOP, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SPOP_p.W131C
FEATURES             Location/Qualifiers
     source          1..3918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      59..647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     548..567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     801..818
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(977..1004)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1096..1182)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1231..1253
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1245..1262
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1246..1262
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1278..1377
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2506..2605)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2622..2641)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2623..2641)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2646..2662)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2646..2662)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3093..3884
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
       61 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
      121 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
      181 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
      241 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
      301 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
      361 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
      421 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      481 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      541 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      601 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      661 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      721 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      781 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      841 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
      901 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
      961 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     1021 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     1081 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     1141 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     1201 ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca
     1261 gtcttaagct cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
     1321 aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gttggcacca
     1381 tgtcaagggt tccaagtcct ccacctccgg cagaaatgtc gagtggcccc gtagctgaga
     1441 gttggtgcta cacacagatc aaggtagtga aattctccta catgtggacc atcaataact
     1501 ttagcttttg ccgggaggaa atgggtgaag tcattaaaag ttctacattt tcatcaggag
     1561 caaatgataa actgaaatgg tgtttgcgag taaaccccaa agggttagat gaagaaagca
     1621 aagattacct gtcactttac ctgttactgg tcagctgtcc aaagagtgaa gttcgggcaa
     1681 aattcaaatt ctccatcctg aatgccaagg gagaagaaac caaagctatg gagagtcaac
     1741 gggcatatag gtttgtgcaa ggcaaagact gtggattcaa gaaattcatc cgtagagatt
     1801 ttcttttgga tgaggccaac gggcttctcc ctgatgacaa gcttaccctc ttctgcgagg
     1861 tgagtgttgt gcaagattct gtcaacattt ctggccagaa taccatgaac atggtaaagg
     1921 ttcctgagtg ccggctggca gatgagttag gaggactgtg ggagaattcc cggttcacag
     1981 actgctgctt gtgtgttgcc ggccaggaat tccaggctca caaggctatc ttagcagctc
     2041 gttctccggt ttttagtgcc atgtttgaac atgaaatgga ggagagcaaa aagaatcgag
     2101 ttgaaatcaa tgatgtggag cctgaagttt ttaaggaaat gatgtgcttc atttacacgg
     2161 ggaaggctcc aaacctcgac aaaatggctg atgatttgct ggcagctgct gacaagtatg
     2221 ccctggagcg cttaaaggtc atgtgtgagg atgccctctg cagtaacctg tccgtggaga
     2281 acgctgcaga aattctcatc ctggccgacc tccacagtgc agatcagttg aaaactcagg
     2341 cagtggattt catcaactat catgcttcgg atgtcttgga gacctctggg tggaagtcaa
     2401 tggtggtgtc acatccccac ttggtggctg aggcataccg ctctctggct tcagcacagt
     2461 gcccttttct gggaccccca cgcaaacgcc tgaagcaatc ctaattgcca actttcttgt
     2521 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     2581 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     2641 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     2701 ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct
     2761 cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     2821 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     2881 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     2941 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     3001 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     3061 agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat
     3121 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     3181 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     3241 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     3301 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     3361 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     3421 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     3481 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     3541 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     3601 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     3661 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     3721 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     3781 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     3841 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
     3901 gaccaaaatc ccttaacg
//
