LOCUS       sequence-166348-        3918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SPOP, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SPOP_p.E47A
FEATURES             Location/Qualifiers
     source          1..3918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      41..629
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     530..549
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     783..800
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(959..986)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1078..1164)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1213..1235
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1227..1244
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1228..1244
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1260..1359
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2488..2587)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2604..2623)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2605..2623)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2628..2644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2628..2644)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3075..3866
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
       61 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
      121 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
      181 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
      241 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
      301 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      361 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      421 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      481 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      541 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      601 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      661 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      721 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      781 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
      841 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
      901 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
      961 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     1021 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     1081 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     1141 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag
     1201 catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccc
     1261 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat
     1321 gcttttttat aatgccaact ttgtacaaaa aagttggcac catgtcaagg gttccaagtc
     1381 ctccacctcc ggcagaaatg tcgagtggcc ccgtagctga gagttggtgc tacacacaga
     1441 tcaaggtagt gaaattctcc tacatgtgga ccatcaataa ctttagcttt tgccgggagg
     1501 caatgggtga agtcattaaa agttctacat tttcatcagg agcaaatgat aaactgaaat
     1561 ggtgtttgcg agtaaacccc aaagggttag atgaagaaag caaagattac ctgtcacttt
     1621 acctgttact ggtcagctgt ccaaagagtg aagttcgggc aaaattcaaa ttctccatcc
     1681 tgaatgccaa gggagaagaa accaaagcta tggagagtca acgggcatat aggtttgtgc
     1741 aaggcaaaga ctggggattc aagaaattca tccgtagaga ttttcttttg gatgaggcca
     1801 acgggcttct ccctgatgac aagcttaccc tcttctgcga ggtgagtgtt gtgcaagatt
     1861 ctgtcaacat ttctggccag aataccatga acatggtaaa ggttcctgag tgccggctgg
     1921 cagatgagtt aggaggactg tgggagaatt cccggttcac agactgctgc ttgtgtgttg
     1981 ccggccagga attccaggct cacaaggcta tcttagcagc tcgttctccg gtttttagtg
     2041 ccatgtttga acatgaaatg gaggagagca aaaagaatcg agttgaaatc aatgatgtgg
     2101 agcctgaagt ttttaaggaa atgatgtgct tcatttacac ggggaaggct ccaaacctcg
     2161 acaaaatggc tgatgatttg ctggcagctg ctgacaagta tgccctggag cgcttaaagg
     2221 tcatgtgtga ggatgccctc tgcagtaacc tgtccgtgga gaacgctgca gaaattctca
     2281 tcctggccga cctccacagt gcagatcagt tgaaaactca ggcagtggat ttcatcaact
     2341 atcatgcttc ggatgtcttg gagacctctg ggtggaagtc aatggtggtg tcacatcccc
     2401 acttggtggc tgaggcatac cgctctctgg cttcagcaca gtgccctttt ctgggacccc
     2461 cacgcaaacg cctgaagcaa tcctaattgc caactttctt gtacaaagtt ggcattataa
     2521 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     2581 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
     2641 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     2701 tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     2761 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     2821 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     2881 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     2941 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     3001 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     3061 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     3121 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     3181 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     3241 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     3301 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     3361 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     3421 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     3481 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     3541 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     3601 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     3661 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     3721 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     3781 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     3841 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     3901 tgagttacgc gtcgttcc
//
