LOCUS       sequence-166372-        3891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing STK19, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_STK19_p.E88K
FEATURES             Location/Qualifiers
     source          1..3891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     63..80
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(239..266)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(358..444)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     493..515
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     507..524
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     508..524
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    540..639
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1741..1840)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1857..1876)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1858..1876)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1881..1897)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1881..1897)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2328..3119
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3212..3800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3701..3720
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
       61 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
      121 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
      181 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
      241 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
      301 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
      361 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
      421 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag
      481 catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccc
      541 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat
      601 gcttttttat aatgccaact ttgtacaaaa aagcaggctt caccatgcaa aagtggtttt
      661 ctgctttcga tgatgcaatc attcagcgac agtggcgggc aaacccctcc cggggcgggg
      721 gaggtgtgag cttcacgaag gaggttgaca ccaacgtggc caccggcgcc cctccacgcc
      781 gccaacgagt ccccgggcgt gcgtgccctt ggagggagcc aatccgcggc cggcgtgggg
      841 cccggcctgg cggaggtgat gctggaggga cgcccgggga gaccgtacgt cactgctctg
      901 cgccgaaaga ccctattttc aggttctctt ccctccattc ctaccccttc cccggtacca
      961 taaaatcccg ggatatgagc tggaagaggc atcacctgat cccggagacc tttggagtta
     1021 agaggcggcg gaagcgaggg cctgtggagt cggatcctct tcggggtgag ccagggtcgg
     1081 cgcgcgcggc tgtctcagaa ctcatgcagc tgttcccgcg aggcctgttt gaggacgcgc
     1141 tgccgcccat cgtgctgagg agccaggtgt acagccttgt gcctgacagg accgtggccg
     1201 accggcagct gaaggagctt caagagcagg gggagatcag aatcgtccag ctgggcttcg
     1261 acttggatgc ccatggaatt atcttcactg aggactacag gaccagagtc ctcaaggcct
     1321 gtgatggccg accgtatgct ggggcagtgc agaaatttct agcttcagta cttccagcct
     1381 gtggggacct tagtttccag caggaccaaa tgacacagac ctttggcttc agggactcag
     1441 aaatcacgca tctggtgaat gctggagtcc tcaccgtccg agatgctggg agctggtggc
     1501 tagctgtgcc tggagctggg agattcatca agtactttgt taaagggcgc caggctgtcc
     1561 ttagcatggt ccggaaggca aagtaccggg aactgctcct atcagagctc ctgggccggc
     1621 gggcgcctgt cgtggtgcgg cttggcctca cctaccatgt gcacgacctc attggggccc
     1681 agctagtgga ctgcatctct accacttcag gaaccctcct ccgcctgcca gagacatgat
     1741 tgccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
     1801 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
     1861 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     1921 atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc
     1981 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
     2041 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     2101 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     2161 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     2221 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     2281 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     2341 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     2401 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     2461 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     2521 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     2581 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     2641 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     2701 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     2761 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     2821 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     2881 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     2941 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     3001 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     3061 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     3121 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     3181 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3241 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3301 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3361 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3421 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3481 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3541 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3601 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3661 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3721 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3781 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3841 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg g
//
