LOCUS       sequence-166533-        7904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 
            scaffold domains + salicylate inducible phosphatase (Psal-CusSmut).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7904)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 7904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH028
FEATURES             Location/Qualifiers
     source          1..7904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2442..2471
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2487..2504
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2730..2776
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             2918..3577
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2984..3003)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      3598..3692
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(4116..4133)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4250..4795)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4330..4349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4868..4914)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(5140..5157)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(5173..5202)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gaattcacta gtttgacagt tagctcagtc ctaggaatta tgctagcggt acctcccggt
       61 gccctctacc agggccaaga gatctatgat taaccgtatc cgcgtagtca cgctgttggt
      121 aatggtgctg ggggtattcg cactgttaca gcttatttcc ggcagtctgt ttttttcttc
      181 ccttcaccat agccagaaga gctttgtggt ttccaatcaa ttacgggaac agcagggcga
      241 gctgacgtca acctgggatt taatgctgca aacgcgcatt aacctgagtc gttcagcggt
      301 acggatgatg atggattcct ccaatcaaca aagtaacgcc aaagttgaat tgctcgatag
      361 cgccaggaaa acattggcgc aggcagcgac gcattataaa aaattcaaaa gcatggcacc
      421 gttacctgaa atggtcgcta ccagtcgtaa tattgatgaa aaatataaaa actattacac
      481 agcgttaact gaactgattg attatctaga ttatggcaat actggagctt atttcgctca
      541 gccaacccag ggaatgcaaa atgcaatggg cgaagcgttt gctcagtacg ccctcagcag
      601 tgaaaaactg tatcgcgata tcgtcactga caacgcagat gattaccgat ttgcccagtg
      661 gcaactggcg gttatcgcgc tggtggtggt attgattctg ctggtggcgt ggtacggcat
      721 tcgccgtatg ttgcttactc cgctggcaaa aattattgct cacattcgcg aaatcgccgg
      781 tggtaacctg gcgaataccc tgaccattga cgggcgcagt gaaatgggcg acctggcgca
      841 gagcgtttca catatggcgg ctggtgttaa gcaactggcg gatgaccgca cgctgctgat
      901 ggcgggggta agtcacgact tgcgcacgcc gctgacgcgt attcgccttg cgactgagat
      961 gatgagcgag caggatggct atctggcaga atcgatcaat aaagatatcg aagagtgcaa
     1021 cgccatcatt gagcagttta tcgactacct gcgcaccggg caggagatgc cgatggaaat
     1081 ggcggatctt aatgcagtac tcggtgaggt gattgctgcc gaaagtggct atgagcggga
     1141 aattgaaacc gcgctttacc ccggcagcat tgaagtgaaa atgcacccgc tgtcgatcaa
     1201 acgcgcggtg gcgaatatgg tggtcaacgc cgcccgttat ggcaatggct ggatcaaagt
     1261 cagcagcgga acggagccga atcgcgcctg gttccaggtg gaagatgacg gtccgggaat
     1321 tgcgccggaa caacgtaagc acctgttcca gccgtttgtc cgcggcgaca gtgcgcgcac
     1381 cattagcggc acgggattag ggctggcaat tgtgcagcgt atcgtggata accataacgg
     1441 gatgctggag cttggcacca gcgagcgggg cgggctttcc attcgcgcct ggctgccagt
     1501 gccggtaacg cgggcgcagg gcacgacaaa agaaggggga tctggtagtg gcagcggcag
     1561 cggtagtgga tctggtagtg gcagcggcag cggtagtgga tctatggcag agtatgtgcg
     1621 ggccctgttt gactttaatg ggaatgatga agaagacctt ccctttaaga aaggagacat
     1681 cctgagaatc cgggataagc ctgaagagca gtggtggaat gcagaggaca gcgaaggaaa
     1741 gagggggatg attcctgtcc cttacgtgga gaagtatgga tctggtagtg gcagcggcag
     1801 cggtagtgga tctatggcag agtatgtgcg ggccctgttt gactttaatg ggaatgatga
     1861 agaagacctt ccctttaaga aaggagacat cctgagaatc cgggataagc ctgaagagca
     1921 gtggtggaat gcagaggaca gcgaaggaaa gagggggatg attcctgtcc cttacgtgga
     1981 gaagtatgga tctggtagtg gcagcggcag cggtagtgga tctatggcag agtatgtgcg
     2041 ggccctgttt gactttaatg ggaatgatga agaagacctt ccctttaaga aaggagacat
     2101 cctgagaatc cgggataagc ctgaagagca gtggtggaat gcagaggaca gcgaaggaaa
     2161 gagggggatg attcctgtcc cttacgtgga gaagtatgga tctggtagtg gcagcggcag
     2221 cggtagtgga tctatggcag agtatgtgcg ggccctgttt gactttaatg ggaatgatga
     2281 agaagacctt ccctttaaga aaggagacat cctgagaatc cgggataagc ctgaagagca
     2341 gtggtggaat gcagaggaca gcgaaggaaa gagggggatg attcctgtcc cttacgtgga
     2401 gaagtatgga tcctgactcg agtaatgaga agcttgggcc cgaacaaaaa ctcatctcag
     2461 aagaggatct gaatagcgcc gtcgaccatc atcatcatca tcattgagtt taaacggtct
     2521 ccagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga
     2581 acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc
     2641 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc
     2701 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     2761 gggcctttcg ttttatctgt tgtttgtcgg tgaactaatt tctagactgc agttgatcgg
     2821 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
     2881 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     2941 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     3001 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     3061 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     3121 tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     3181 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     3241 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     3301 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     3361 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     3421 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     3481 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     3541 acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac
     3601 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg
     3661 gttgccgccg ggcgtttttt attggtgaga atccaagcct cggtgagaat ccaagcctcg
     3721 atcaacgtct cattttcgcc aaaagttggc ccagggcttc ccggtatcaa cagggacacc
     3781 aggatttatt tattctgcga agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc
     3841 gtcgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag
     3901 tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg
     3961 gcaaaagcac cgccggacat cagcggtagc ggagtgtata ctggcttact atgttggcac
     4021 tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt
     4081 cagcagaata tgtgatacag gatatattcc gcttcctcgc tcactgactc gctacgctcg
     4141 gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg gcggagattt cctggaagat
     4201 gccaggaaga tacttaacag ggaagtgaga gggccgcggc aaagccgttt ttccataggc
     4261 tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga
     4321 caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc
     4381 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc
     4441 ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc
     4501 gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga
     4561 catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa
     4621 gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag
     4681 ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa
     4741 ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga
     4801 tcatcttatt aatcagataa aatatttaat ttctagactg cagttgaagt tcaccgacaa
     4861 acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct
     4921 ggcagttccc tactctcgca tggggagacc ccacactacc atcggcgcta cggcgtttca
     4981 cttctgagtt cggcatgggg tcaggtggga ccaccgcgct actgccgcca ggcaaattct
     5041 gttttatcag accgcttctg cgttctgatt taatctgtat caggctgaaa atcttctctc
     5101 atccgccaaa acagccaagc tggagaccgt ttaaactcaa tgatgatgat gatgatggtc
     5161 gacggcgcta ttcagatcct cttctgagat gagtttttgt tcgggcccaa gcttctcatt
     5221 actcgagtta ggatccagcg ggtaatgtga taacaaacct tgtcccccgc gcatccgacg
     5281 ttaccgcaac cgtgccttta tgcgcgacaa caatcgattt cactatcgcc agcgcaatac
     5341 cgctaccttc accttttcgc tggcgggagg ggtcaacgcg atagaaacgg tcaaacaatc
     5401 gcggtaagtg ctcgggcgca atgggcgtac cggggttttc gacgataact tgcaccaggt
     5461 gatcgaccgt ctgacagcgc actacaattg tctctccggt tggcgtataa cgcagggcgt
     5521 tcgaaagcag gttgcttagc gcccgacgca gcatcagcgg atcgcccgcg acctgacact
     5581 tgtcgccaac aaaccgcaac tccacgccgc gatcttccgc taacgcctcg aaaaaatcga
     5641 acactttgcc gacttcatcc gccaggttga gcattttctt ttcggggatt agctggttgt
     5701 tatcggcctg agcgagaaac agcatatcgc tgaccatttt cgccattcgc gtcagctctt
     5761 cgagattaga gtagagcaca tcttccagct ccttctggct gcgcgactgg ctgagggcga
     5821 tttcggtttg cgttatgaga ttcgtaattg gtgtgcgaat ttcgtgagcg atatccgctg
     5881 agaaattgga ctggcgggta aagacatcct caatacgctc gatcatatgg ttgaacgaca
     5941 gtaccagctg ttccagctca atgggcacgg tctgcgggtc gaggcgaacg tcgagatcct
     6001 tcgaggtaat attctggatt tgacggctga cgctgcggat cggcgcgtga cctttatgta
     6061 ccgccaacag tacgataaag acgatcagga tgctgattac cgatgcggtc ataataagtt
     6121 tattcatcaa atcatttatg taatgaagat gaaaatcgat cgaaagcgcg atgtagagcg
     6181 tataaatcgg tttgccgtcc accaacgggc caaccggcaa gttaatcatc cgccagttgc
     6241 tgtgttccat atgcccgtga ccgtggcctg gcatcatcat cgtcgggccg gaaaggagat
     6301 acacctcgcc accctgagcg tctttatcgg gtatggcgtc acgcgtaaac tcgcggatat
     6361 ccggcgcacc gggggagtga tacaccgttt taccctgact atctgccagg gaaatcaaca
     6421 cattggaata accactgacg atatcttcca gcgtcattaa gcgtcgggct tgcgtttcgt
     6481 cagggtgatt tagtacccgt tcaagggtgg cgctaatctc ttttaaatca ttaatatcct
     6541 gctcggcaaa atgaactttt actgagtgga tcatgatcca tgcaaaggcg aaaaacgccg
     6601 cgatggtggc caggctgata aaaaaggtca ggcgggttgc cagcgaaaac gggcgctgaa
     6661 atggcttact gaccatagat ctttatattg tgaggtaccg ctagctctat ggtactcgtg
     6721 atggctttat tgatgacttg ttaataacga taacggagca aacaatattg ataaatacac
     6781 cactcgatat ataataaatc atcaacatga atattgcgcc cggccgggca ccagcaataa
     6841 cccaagcgag gccccatgga actgcgtgac ctggatttaa acctgctggt ggtgttcaac
     6901 caattgctgg tcgacagacg cgtctctatc actgcggaga acctgggcct gacccagcct
     6961 gccgtgagca atgcgctgaa acgcctgcgc acctcgctac aggacccact cttcgtgcgc
     7021 acacatcagg gaatggaacc cacaccctat gccgcgcatc tggccgagcc cgtcacttcg
     7081 gccatgcacg cactgcgcaa cgccctacag caccatgaaa gcttcgatcc gctgaccagc
     7141 gagcgtacct tcaccctggc catgaccgac attggcgaaa tctacttcat gccgcggctg
     7201 atggatgtgc tggctcacca ggcccccaat tgcgtgatca gtacggtgcg cgacagttcg
     7261 atgagcctga tgcaggcctt gcagaacgga accgtggact tggccgtggg cctgcttccc
     7321 aatctgcaaa ctggcttctt tcagcgccgg ctgctccaga atcactacgt gtgcctatgt
     7381 cgcaaggacc atccagtcac ccgcgaaccc ctgactctgg agcgcttctg ttcctacggc
     7441 cacgtgcgtg tcatcgccgc tggcaccggc cacggcgagg tggacacgta catgacacgg
     7501 gtcggcatcc ggcgcgacat ccgtctggaa gtgccgcact tcgccgccgt tggccacatc
     7561 ctccagcgca ccgatctgct cgccactgtg ccgatacgtt tagccgactg ctgcgtggag
     7621 cccttcggcc taagcgcctt gccgcaccca gtcgtcttgc ctgaaatagc catcaacatg
     7681 ttctggcata cgaagtacca caaggaccta gccaatattt ggttgcggca actgatgttt
     7741 gacctgttta cggattgact agatttcagt gcaatttatc tcttcaaatg tagcacctga
     7801 agtcagcccc atacgatata agttgtaatt ctcatgtttg acagcttatc atcgataagc
     7861 ttccgatggc gcgccgagag gctttacact ttatgcttcc ggct
//
