LOCUS       sequence-166585-       14626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drosophila P-element transgenesis vector for cloning rescue 
            transgenes. Cloning site is flanked by FRT sequences and contains 
            constitutive RFP reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14626)
  AUTHORS   Zhou Q, Neal SJ, Pignoni F
  TITLE     Mutant Analysis by Rescue Gene Excision: New tools for mosaic 
            studies in Drosophila.
  JOURNAL   Genesis. 2016 Oct 3. doi: 10.1002/dvg.22984.
  PUBMED    27696669
REFERENCE   2  (bases 1 to 14626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2016 Oct 3. doi: 10.1002/dvg.22984."
COMMENT     Sequence Label: FoRC.PRa
FEATURES             Location/Qualifiers
     source          1..14626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(820..1408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(900..919)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1579..2370,2371..2439))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2202..2221
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2440..2544)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2612..2630
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2668..2690)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2790..2809
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(3346..3376,3377..3578)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3765..3812
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    4060..4107
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6008..6682
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(6138..6156)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(6188..6208)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     6406..6425
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6600..6623
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     7325..7341
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7325..7341
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7346..7365
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7346..7364
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7383..7614
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7759..7861
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             7862..8545
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     complement(7928..7947)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8865..9170
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9049..9068
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(9566..9797)
                     /gene="mutant version of attP"
                     /label=attP1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9814..9831)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9814..9830)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9981..10028
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    10276..10323
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     gene            10488..14615
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(10497..10515)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     misc_feature    join(14626,1..554,555..585)
                     /label=P element 5' end
ORIGIN
        1 ctgctgctct aaacgacgca tttcgtactc caaagtacga attttttccc tcaagctctt
       61 attttcatta aacaatgaac aggacctaac gcacagtcac gttattgttt acataaatga
      121 ttttttttac tattcaaact tactctgttt gtgtactccc actggtatag ccttctttta
      181 tcttttctgg ttcaggctct atcactttac taggtacggc atctgcgttg agtcgcctcc
      241 ttttaaatgt ctgacctttt gcaggtgcag ccttccactg cgaatcttta aagtgggtat
      301 cacaaatttg ggagttttca ccaaggctgc acccaaggct ctgctcccac aattttctct
      361 taatagcaca cttcggcacg tgaattaatt ttactccagt cacagctttg cagcaaaatt
      421 tgcaatattt catttttttt tattccacgt aagggttaat gttttcaaaa aaaaattcgt
      481 ccgcacacaa cctttcctct caacaagcaa acgtgcactg aatttaagtg tatacttcgg
      541 taagcttcgg ctatcgacgg gaccacctta tgttatttca tcatgggcca gacccacgta
      601 gtccagcggc agatcggcgg cggagaagtt aagcgtctcc aggatgacct tgcccgaact
      661 ggggcacgtg gtgttcgacg atgtgcagct aatttcgccc ggctccacgt ccgcccattg
      721 gttaatcagc agaccctcgt tggcgtaacg gaaccatgag aggtacgaca accatttgag
      781 gtatactggc accgagcccg agttcaagaa gaaggcgttt ttccataggc tccgcccccc
      841 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      901 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      961 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     1021 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1081 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1141 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1201 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1261 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1321 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1381 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1441 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1501 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1561 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1621 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1681 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1741 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1801 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1861 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     1921 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     1981 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2041 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2101 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2161 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2221 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2281 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2341 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2401 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2461 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2521 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     2581 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     2641 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     2701 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     2761 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     2821 ccatatgcgg tgtgaaatac cgcaccgaat cgcgcggaac taacgacagt cgctccaagg
     2881 tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact
     2941 acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg cgcgatttga aaagtatgta
     3001 tataaaaaat atatcccggt gttttatgta gcgataaacg agtttttgat gtaaggtatg
     3061 caggtgtgta agtcttttgg ttagaagaca aatccaaagt ctacttgtgg ggatgttcga
     3121 aggggaaata cttgtattct ataggtcata tcttgttttt attggcacaa atataattac
     3181 attagctttt tgagggggca ataaacagta aacacgatgg taataatggt aaaaaaaaaa
     3241 acaagcagtt atttcggata tatgtcggct actccttgcg tcgggcccga agtcttagag
     3301 ccagatatgc gagcacccgg aagctcacga tgagaatggc cagaccatga tgaaataaca
     3361 taaggtggtc ccgtcggcaa gagacatcca cttaacgtat gcttgcaata agtgcgagtg
     3421 aaaggaatag tattctgagt gtcgtattga gtctgagtga gacagcgata tgattgttga
     3481 ttaaccctta gcatgtccgt ggggtttgaa ttaactcata atattaatta gacgaaatta
     3541 tttttaaagt tttattttta ataatttgcg agtacgcaaa gcttggctgc aggtcgactc
     3601 tagaggatct ggtctagagc ccgggcgaat tcacgcacta tgccgttctt ctcatgtata
     3661 tatatataca ggcaacacgc agatataggt gcgacgtgaa cagtgagctg tatgtgcgca
     3721 gctcgcgttg cattttcgga agcgctcgtt ttcggaaacg ctttgaagtt cctattccga
     3781 agttcctatt ctctagaaag tataggaact tcagagcgct tttgaaaacc aaaagcgctc
     3841 tgaagacgca ctttcaaaaa accaaaaacg caccggactg taacgagcta ctaaaatatt
     3901 gcgaataccg cttccacaaa cgaattcacg cactatgccg ttcttctcat gtatatatat
     3961 atacaggcaa cacgcagata taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg
     4021 cgttgcattt tcggaagcgc tcgttttcgg aaacgctttg aagttcctat tccgaagttc
     4081 ctattctcta gaaagtatag gaacttcaga gcgcttttga aaaccaaaag cgctctgaag
     4141 acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa atattgcgaa
     4201 taccgcttcc acaaacgaat tcgccggcgc gccggtaccc cactgtccaa aatcgaaaat
     4261 cgccgaaccg tagtgtgacc gtgcggggct ctgcgaaaat aaactttttt aggtatatgg
     4321 ccacacacgg ggaaagcaca gtggattata tgttttaata ttataatatg caggttttca
     4381 ttacttatcc agatgtaagc ccacttaaag cgatttaaca attatttgcc gaaagagtat
     4441 aaacaaattt cacttaaaaa tggattaaga aaagcttgtg taagattatg cgcagcgttg
     4501 ccagatagct ccatttaaaa cacttcaaaa acaataagtt ttgaaaatat atacataaat
     4561 agcagtcgtt gccgcaacgc tcaacacatc acacttttaa aacacccttt acctacacag
     4621 aattactttt taaatttcca gtcaagctgc gagtttcaaa attatagccg gtagagaaga
     4681 cagtgctatt tcaaaagcaa actaacaagg gtcttaaatt ccaaaacacc aatcctaaca
     4741 agccttggac ttttgtaagt ttagatcaaa ggtggcattg cattcaatgt catggtaaga
     4801 agtaggtcgt ctaggtagaa atcctcattc agccggtcaa gtcagtacga gaaaggtctc
     4861 aatttgaaat tgtcttaaaa atattttatt gttttgtact gtggtgagtt taaacgaaaa
     4921 acacaaaaaa aaagtgatac acagaaatca taaaaaattt taatacaagg tattcgtacg
     4981 tatcaaaaac atttcggcac aatttttttt ctctgtacta aagtgttacg aacactacgg
     5041 tattttttag tgattttcaa cggacaccga aggtatataa acagcgttcg cgaacggtcg
     5101 ccttcaaaac caattgacat ttgcagcagc aagtacaagt agaaagtaaa gcgcaatcag
     5161 cgaaaaattt atacttaatt gttggtgatt aaagtacaat taaaagaaca ttctcgaaag
     5221 tcacaagaaa cgtaagtttt taactcgctg ttaccaatta gtaataagag caacaagacg
     5281 ttgagtaatt tcaagaaaaa ctgaatttca aggtctttgt tcggccattt tttttttatt
     5341 caacgctcta cgtaattaca aaataagaaa ttggcagcca cgcatcttgt tttcccaatg
     5401 aattggcatc aaaacgcaaa caaatctata aataaaactt gcgtgttgat tttcgccaag
     5461 atttattggc aaattgtgaa attcgcagtg acgcatttga aaattcgaga aatcacgaac
     5521 gcactcgagc atttgtgtgc atgttattag ttagttgttt agttaattga agtattttac
     5581 caacgaaatc cacttatttt tagctgaaat agagtaggtt gcttaaacaa agccacgtct
     5641 gaaaatttct tattgcttgt agttgtgacg tcaccatata cacacaaaat aatgtgtatg
     5701 catgcatttc agctgtgtat atatacatgc acacactcgc aacacgaaaa cgatgacgaa
     5761 gcaacggaac aaaggtttct caactaccct ttgttccctg tttcttcgct ttcctttgtt
     5821 ccaatattcg tagagggtta ataggggttt ctcaacaaag ttggcgtcga taaataagtt
     5881 tcccattttt attccccagc caggaagtta gtttcaatag ttttgtaatt tcaacgaaac
     5941 tcatttgatt tcgtactaat tttccacatc tctattttct gcccgcagaa taatccaaag
     6001 cgatcgcatg gcctcctccg aggacgtcat caaggagttc atgcgcttca aggtgcgcat
     6061 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     6121 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     6181 ggacatcctg tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga
     6241 catccccgac tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     6301 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggctgctt
     6361 catctacaag gtgaagttca tcggcgtgaa cttcccctcc gacggccccg taatgcagaa
     6421 gaagactatg ggctgggagc cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
     6481 gggcgagatc cacaaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
     6541 gtccatctac atggccaaga agcccgtgca gctgcccggc tactactacg tggactccaa
     6601 gctggacatc acctcccaca acgaggacta caccatcgtg gagcagtacg agcgcgccga
     6661 gggccgccac cacctgttcc tgtacaagag caggcacaga aggcatcgcc agcgctctag
     6721 gagccgcaat cgcagccgaa gtcgcagcag tgaacgaaaa cgccgtcaac ggagccgaag
     6781 tcgcagcagt gaacgaagac gctacttgta cagcggccgg ccgcgactct aggcgatcgc
     6841 gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggg
     6901 gggatcgatc ccccagatcc gtacgatcct ctagtaaaaa aaaaacaaaa ttttagtgtg
     6961 tataattagg ctaaacaact ggaggattca ttgctccctt tgagtttata ctttttgtaa
     7021 caagaacagg cgcaacactc gggagaattt tgcatttcat ttgtgaataa acgaatacaa
     7081 taaatacaaa tacacgaaaa gaagtcagct gtcgagtttt tgttggtttt tttttccctt
     7141 tttagcttcg aatctgcaaa tgtatttgca tttaagtaaa ccggcttttg ttgggtgtcg
     7201 ttaactttca aggggtctta tgtacttatt ctgttttcct tgtccttgaa ttggacttgt
     7261 ggaaaattaa atggtcagcc aattgcttaa cctttgtcga cctgcggccg cggtggccgg
     7321 ccgccaggaa acagctatga ccatgtaata cgactcacta taggggatat cagctggatg
     7381 gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca
     7441 atgctttctt ataatgccaa ctttgtacaa gaaagctgaa cgagaaacgt aaaatgatat
     7501 aaatatcaat atattaaatt agactttgca taaaaaacag actacataat actgtaaaac
     7561 acaacatatc cagtcactat gaatcaacta cttagatggt attagtgacc tgtagtcgac
     7621 taagttggca gcatcacccg acgcactttg cgccgaataa atacctgtga cggaagatca
     7681 cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct gggccaactt
     7741 ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat
     7801 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
     7861 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     7921 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     7981 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     8041 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
     8101 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     8161 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     8221 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     8281 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     8341 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     8401 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     8461 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gggggcgtaa
     8521 acgccgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat
     8581 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg
     8641 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat
     8701 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct
     8761 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat
     8821 tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt
     8881 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     8941 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     9001 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     9061 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc
     9121 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     9181 cccttataca cagccagtct gcaggtcgat acagtagaaa ttacagaaac tttatcacgt
     9241 ttagtaagta tagaggctga aaatccagat gaagccgaac gacttgtaag agaaaagtat
     9301 aagagttgtg aaattgttct tgatgcagat gattttcagg actatgacac tagcgtatat
     9361 gaataggtag atgtttttat tttgtcacac aaaaaagagg ctcgcacctc tttttcttat
     9421 ttctttttat gatttaatac ggcattgagg acaatagcga gtaggctgga tacgacgatt
     9481 ccgtttgaga agaacatttg gaaggctgtc ggtcgactaa gttggcagca tcacccgaag
     9541 aacatttgga aggctgtcgg tcgactacag gtcactaata ccatctaagt agttgattca
     9601 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
     9661 atttaatata ttgatattta tatcatttta cgtttctcgt tcagcttttt tgtacaaagt
     9721 tggcattata aaaaagcatt gctcatcaat ttgttgcaac gaacaggtca ctatcagtca
     9781 aaataaaatc attatttggg gcccgagctt aagactggcc gtcgttttac aggccggcct
     9841 cctgcaggac gcactatgcc gttcttctca tgtatatata tatacaggca acacgcagat
     9901 ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc gcgttgcatt ttcggaagcg
     9961 ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt cctattctct agaaagtata
    10021 ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa gacgcacttt caaaaaacca
    10081 aaaacgcacc ggactgtaac gagctactaa aatattgcga ataccgcttc cacaaacgaa
    10141 ttcacgcact atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg
    10201 tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt
    10261 tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac
    10321 ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac
    10381 gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa acgaattcgc
    10441 tagcttaatc actagtgaat tcgcggccgc agatctgtta acgaattcta gtatgtatgt
    10501 aagttaataa aacccatttt tgcggaaagt agataaaaaa aacatttttt ttttttactg
    10561 cactggatat cattgaactt atctgatcag ttttaaattt acttcgatcc aagggtattt
    10621 gatgtaccag gttctttcga ttacctctca ctcaaaatga cattccactc aaagtcagcg
    10681 ctgtttgcct ccttctctgt ccacagaaat atcgccgtct ctttcgccgc tgcgtccgct
    10741 atctctttcg ccaccgtttg tagcgttacc tagcgtcaat gtccgccttc agttgcactt
    10801 tgtcagcggt ttcgtgacga agctccaagc ggtttacgcc atcaattaaa cacaaagtgc
    10861 tgtgccaaaa ctcctctcgc ttcttatttt tgtttgtttt ttgagtgatt ggggtggtga
    10921 ttggttttgg gtgggtaagc aggggaaagt gtgaaaaatc ccggcaatgg gccaagagga
    10981 tcaggagcta ttaattcgcg gaggcagcaa acacccatct gccgagcatc tgaacaatgt
    11041 gagtagtaca tgtgcataca tcttaagttc acttgatcta taggaactgc gattgcaaca
    11101 tcaaattgtc tgcggcgtga gaactgcgac ccacaaaaat cccaaaccgc aatcgcacaa
    11161 acaaatagtg acacgaaaca gattattctg gtagctgtgc tcgctatata agacaatttt
    11221 taagatcata tcatgatcaa gacatctaaa ggcattcatt ttcgactaca ttctttttta
    11281 caaaaaatat aacaaccaga tattttaagc tgatcctaga tgcacaaaaa ataaataaaa
    11341 gtataaacct acttcgtagg atacttcgtt ttgttcgggg ttagatgagc ataacgcttg
    11401 tagttgatat ttgagatccc ctatcattgc agggtgacag cggacgcttc gcagagctgc
    11461 attaaccagg gcttcgggca ggccaaaaac tacggcacgc tcctgccacc cagtccgccg
    11521 gaggactccg gttcagggag cggccaacta gccgagaacc tcacctatgc ctggcacaat
    11581 atggacatct ttggggcggt caatcagccg ggctccggat ggcggcagct ggtcaaccgg
    11641 acacgcggac tattctgcaa cgagcgacac ataccggcgc ccaggaaaca tttgctcaag
    11701 aacggtgagt ttctattcgc agtcggctga tctgtgtgaa atcttaataa agggtccaat
    11761 taccaatttg aaactcagtt tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg
    11821 gcagttccgg tgccggaaag acgaccctgc tgaatgccct tgcctttcga tcgccgcagg
    11881 gcatccaagt atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg
    11941 agatgcaggc caggtgcgcc tatgtccagc aggatgacct ctttatcggc tccctaacgg
    12001 ccagggaaca cctgattttc caggccatgg tgcggatgcc acgacatctg acctatcggc
    12061 agcgagtggc ccgcgtggat caggtgatcc aggagctttc gctcagcaaa tgtcagcaca
    12121 cgatcatcgg tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg
    12181 cattcgcctc cgaggcacta accgatccgc cgcttctgat ctgcgatgag cccacctccg
    12241 gactggactc atttaccgcc cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg
    12301 gcaagaccgt catcctgacc attcatcagc cgtcttccga gctgtttgag ctctttgaca
    12361 agatccttct gatggccgag ggcagggtag ctttcttggg cactcccagc gaagccgtcg
    12421 acttcttttc ctagtgagtt cgatgtgttt attaagggta tctagcatta cattacatct
    12481 caactcctat ccagcgtggg tgcccagtgt cctaccaact acaatccggc ggacttttac
    12541 gtacaggtgt tggccgttgt gcccggacgg gagatcgagt cccgtgatcg gatcgccaag
    12601 atatggcgac aattttgcta ttagcaaagt agcccgggat atggagcagt tgttggccac
    12661 caaaaatttg gagaagccac tggagcagcc ggagaatggg tacacctaca aggccacctg
    12721 gttcatgcag ttccgggcgg tcctgtggcg atcctggctg tcggtgctca aggaaccact
    12781 cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt tccagtggaa acaaatgata
    12841 taacgcttac aattcttgga aacaaattcg ctagatttta gttagaattg cctgattcca
    12901 cacccttctt agtttttttc aatgagatgt atagtttata gttttgcaga aaataaataa
    12961 atttcattta actcgcgaac atgttgaaga tatgaatatt aatgagatgc gagtaacatt
    13021 ttaatttgca gatggttgcc atcttgattg gcctcatctt tttgggccaa caactcacgc
    13081 aagtgggcgt gatgaatatc aacggagcca tcttcctctt cctgaccaac atgacctttc
    13141 aaaacgtctt tgccacgata aatgtaagtc ttgtttagaa tacatttgca tattaataat
    13201 ttactaactt tctaatgaat cgattcgatt taggtgttca cctcagagct gccagttttt
    13261 atgagggagg cccgaagtcg actttatcgc tgtgacacat actttctggg caaaacgatt
    13321 gccgaattac cgctttttct cacagtgcca ctggtcttca cggcgattgc ctatccgatg
    13381 atcggactgc gggccggagt gctgcacttc ttcaactgcc tggcgctggt cactctggtg
    13441 gccaatgtgt caacgtcctt cggatatcta atatcctgcg ccagctcctc gacctcgatg
    13501 gcgctgtctg tgggtccgcc ggttatcata ccattcctgc tctttggcgg cttcttcttg
    13561 aactcgggct cggtgccagt atacctcaaa tggttgtcgt acctctcatg gttccgttac
    13621 gccaacgagg gtctgctgat taaccaatgg gcggacgtgg agccgggcga aattagctgc
    13681 acatcgtcga acaccacgtg ccccagttcg ggcaaggtca tcctggagac gcttaacttc
    13741 tccgccgccg atctgccgct ggactacgtg ggtctggcca ttctcatcgt gagcttccgg
    13801 gtgctcgcat atctggctct aagacttcgg gcccgacgca aggagtagcc gacatatatc
    13861 cgaaataact gcttgttttt ttttttacca ttattaccat cgtgtttact gtttattgcc
    13921 ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg acctatagaa
    13981 tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc ttctaaccaa
    14041 aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca taaaacaccg
    14101 ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa ttcacctcca
    14161 ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg caacacattc
    14221 accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc ggttcgctca
    14281 aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat ttgtatgtac
    14341 aatttatttg ctccaatata tttgtatata tttccctcac agctatattt attctaattt
    14401 aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta gcgttacaat
    14461 ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca aaaaaatggt
    14521 gggcataata gtgttgttta tatatatcaa aaataacaac tataataata agaatacatt
    14581 taatttagaa aatgcttgga tttcactgga actagaatta attcgg
//
