LOCUS       sequence-166591-        9471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker 
            fusion. Also expresses Csy4 for cleavage of multiplexed gRNA 
            transcripts. Backbone derived from pSQT1601 (Addgene #53369)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Havlicek S, Shen Y, Alpagu Y, Bruntraeger MB, Zufir NB, Phuah ZY, Fu
            Z, Dunn NR, Stanton LW
  TITLE     Re-engineered RNA-Guided FokI-Nucleases for Improved Genome Editing 
            in Human Cells.
  JOURNAL   Mol Ther. 2017 Feb 1;25(2):342-355. doi: 
            10.1016/j.ymthe.2016.11.007.
  PUBMED    28153087
REFERENCE   2  (bases 1 to 9471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymthe.2016.11"; journalName: "Mol Ther"; date: 
            "2017-02-1- 1"; volume: "25"; issue: "2"; pages: "342-355"
COMMENT     Sequence Label: pSH-Csy4-T2A-SaRFN
FEATURES             Location/Qualifiers
     source          1..9471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1678..1697
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1763..1772
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2333..2386
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2390..2410
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2417..3004
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     CDS             6269..6316
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6323..6403
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(6493..6512)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6558..6613
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6612..6631)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6974..6990)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6974..6990)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6987..7009)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6998..7014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7022..7028,7029..7046,7047..7052))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7067..7088
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7147..7342
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7193..7328
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7348..7482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7385..7404)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7439..7458
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7550..7567)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7721..8309)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7801..7820)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8480..9271,9272..9340))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9103..9122
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9341..9445)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc
     1741 gcgggcccgg gatccaccgg tcgccaccat gggtgatcat tatctggata ttcggctgag
     1801 gcctgatcca gagttcccac ctgcgcagct gatgtctgtc ctttttggca aacttcatca
     1861 ggccctggtt gcccagggcg gagatcggat aggggtaagc tttccagacc tcgacgaaag
     1921 ccggagccgc ctgggagaac gcctgcggat ccacgcttct gccgacgatc tgagagcctt
     1981 gctggcaagg ccatggcttg aggggctccg ggatcacctg cagtttggcg aacccgccgt
     2041 tgttccccac ccaacccctt atcggcaggt gtctagagtg caggccaaat ctaatccaga
     2101 acggctgcga cggcgactca tgcggcgaca tgatcttagc gaggaagagg cccgaaaaag
     2161 aatccctgat accgtggccc gcgcccttga cttgcctttt gtcacactgc ggtcccagag
     2221 tacggggcag catttcagac ttttcattcg acacgggcca ctgcaagtta ccgccgaaga
     2281 aggaggcttt acttgttatg gactctccaa gggaggtttc gtgccctggt ttgagggcag
     2341 aggaagtctg ttaacatgcg gtgacgtcga ggagaatcct ggcccaatgc ctaagaagaa
     2401 gcggaaggtg agcagccaac ttgtgaagtc tgaactcgag gagaaaaaat cagagttgag
     2461 acacaagttg aagtacgtgc cacacgaata catcgagctt atcgagatcg ccagaaacag
     2521 tacccaggat aggatccttg agatgaaagt catggagttc tttatgaagg tctacggtta
     2581 tagaggaaag caccttggcg gtagcagaaa gcccgatggc gccatctata ctgtcggatc
     2641 tcctatcgat tatggggtga tcgtggatac caaagcttac tcaggcgggt acaacttgcc
     2701 cataggacaa gccgacgaga tgcagcggta tgtcgaagag aaccagacgc gcaacaagca
     2761 catcaacccc aatgaatggt ggaaagtgta cccaagtagt gtgactgagt tcaagttcct
     2821 gtttgtctcc ggccacttta agggcaatta taaagctcag ctcactagac tcaatcacat
     2881 cacaaactgc aacggagctg tgttgtcagt ggaggagctc ctgattggag gcgagatgat
     2941 caaagccggc acccttacac tggaggaggt gcggcggaag ttcaacaatg gagagatcaa
     3001 cttcggcgga agcgccggag gcagtggcag cgggagcagt ggtggaagca gtggagctag
     3061 cggcactggc acagcgggcg gaactgggag cggcagtggc acaggtagtg gaaagcggaa
     3121 ctacatcctg ggcctggcca tcggcatcac cagcgtgggc tacggcatca tcgactacga
     3181 gacacgggac gtgatcgatg ccggcgtgcg gctgttcaaa gaggccaacg tggaaaacaa
     3241 cgagggcagg cggagcaaga gaggcgccag aaggctgaag cggcggaggc ggcatagaat
     3301 ccagagagtg aagaagctgc tgttcgacta caacctgctg accgaccaca gcgagctgag
     3361 cggcatcaac ccctacgagg ccagagtgaa gggcctgagc cagaagctga gcgaggaaga
     3421 gttctctgcc gccctgctgc acctggccaa gagaagaggc gtgcacaacg tgaacgaggt
     3481 ggaagaggac accggcaacg agctgtccac caaagagcag atcagccgga acagcaaggc
     3541 cctggaagag aaatacgtgg ccgaactgca gctggaacgg ctgaagaaag acggcgaagt
     3601 gcggggcagc atcaacagat tcaagaccag cgactacgtg aaagaagcca aacagctgct
     3661 gaaggtgcag aaggcctacc accagctgga ccagagcttc atcgacacct acatcgacct
     3721 gctggaaacc cggcggacct actatgaggg acctggcgag ggcagcccct tcggctggaa
     3781 ggacatcaaa gaatggtacg agatgctgat gggccactgc acctacttcc ccgaggaact
     3841 gcggagcgtg aagtacgcct acaacgccga cctgtacaac gccctgaacg acctgaacaa
     3901 tctcgtgatc accagggacg agaacgagaa gctggaatat tacgagaagt tccagatcat
     3961 cgagaacgtg ttcaagcaga agaagaagcc caccctgaag cagatcgcca aagaaatcct
     4021 cgtgaacgaa gaggatatta agggctacag agtgaccagc accggcaagc ccgagttcac
     4081 caacctgaag gtgtaccacg acatcaagga cattaccgcc cggaaagaga ttattgagaa
     4141 cgccgagctg ctggatcaga ttgccaagat cctgaccatc taccagagca gcgaggacat
     4201 ccaggaagaa ctgaccaatc tgaactccga gctgacccag gaagagatcg agcagatctc
     4261 taatctgaag ggctataccg gcacccacaa cctgagcctg aaggccatca acctgatcct
     4321 ggacgagctg tggcacacca acgacaacca gatcgctatc ttcaaccggc tgaagctggt
     4381 gcccaagaag gtggacctgt cccagcagaa agagatcccc accaccctgg tggacgactt
     4441 catcctgagc cccgtcgtga agagaagctt catccagagc atcaaagtga tcaacgccat
     4501 catcaagaag tacggcctgc ccaacgacat cattatcgag ctggcccgcg agaagaactc
     4561 caaggacgcc cagaaaatga tcaacgagat gcagaagcgg aaccggcaga ccaacgagcg
     4621 gatcgaggaa atcatccgga ccaccggcaa agagaacgcc aagtacctga tcgagaagat
     4681 caagctgcac gacatgcagg aaggcaagtg cctgtacagc ctggaagcca tccctctgga
     4741 agatctgctg aacaacccct tcaactatga ggtggaccac atcatcccca gaagcgtgtc
     4801 cttcgacaac agcttcaaca acaaggtgct cgtgaagcag gaagaagcca gcaagaaggg
     4861 caaccggacc ccattccagt acctgagcag cagcgacagc aagatcagct acgaaacctt
     4921 caagaagcac atcctgaatc tggccaaggg caagggcaga atcagcaaga ccaagaaaga
     4981 gtatctgctg gaagaacggg acatcaacag gttctccgtg cagaaagact tcatcaaccg
     5041 gaacctggtg gataccagat acgccaccag aggcctgatg aacctgctgc ggagctactt
     5101 cagagtgaac aacctggacg tgaaagtgaa gtccatcaat ggcggcttca ccagctttct
     5161 gcggcggaag tggaagttta agaaagagcg gaacaagggg tacaagcacc acgccgagga
     5221 cgccctgatc attgccaacg ccgatttcat cttcaaagag tggaagaaac tggacaaggc
     5281 caaaaaagtg atggaaaacc agatgttcga ggaaaagcag gccgagagca tgcccgagat
     5341 cgaaaccgag caggagtaca aagagatctt catcaccccc caccagatca agcacattaa
     5401 ggacttcaag gactacaagt acagccaccg ggtggacaag aagcctaata gagagctgat
     5461 taacgacacc ctgtactcca cccggaagga cgacaagggc aacaccctga tcgtgaacaa
     5521 tctgaacggc ctgtacgaca aggacaatga caagctgaaa aagctgatca acaagagccc
     5581 cgaaaagctg ctgatgtacc accacgaccc ccagacctac cagaaactga agctgattat
     5641 ggaacagtac ggcgacgaga agaatcccct gtacaagtac tacgaggaaa ccgggaacta
     5701 cctgaccaag tactccaaaa aggacaacgg ccccgtgatc aagaagatta agtattacgg
     5761 caacaaactg aacgcccatc tggacatcac cgacgactac cccaacagca gaaacaaggt
     5821 cgtgaagctg tccctgaagc cctacagatt cgacgtgtac ctggacaatg gcgtgtacaa
     5881 gttcgtgacc gtgaagaatc tggatgtgat caaaaaagaa aactactacg aagtgaatag
     5941 caagtgctat gaggaagcta agaagctgaa gaagatcagc aaccaggccg agtttatcgc
     6001 ctccttctac aacaacgatc tgatcaagat caacggcgag ctgtatagag tgatcggcgt
     6061 gaacaacgac ctgctgaacc ggatcgaagt gaacatgatc gacatcacct accgcgagta
     6121 cctggaaaac atgaacgaca agaggccccc caggatcatt aagacaatcg cctccaagac
     6181 ccagagcatt aagaagtaca gcacagacat tctgggcaac ctgtatgaag tgaaatctaa
     6241 gaagcaccct cagatcatca aaaagggcaa aaggccggcg gccacgaaaa aggccggcca
     6301 ggcaaaaaag aaaaagggat cctacccata cgatgttcca gattacgctt acccatacga
     6361 tgttccagat tacgcttacc catacgatgt tccagattac gcttaaagcg gccgcactcc
     6421 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
     6481 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     6541 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     6601 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     6661 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     6721 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     6781 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     6841 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     6901 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
     6961 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7021 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7081 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7141 ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7201 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7261 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7321 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa
     7381 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7441 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg
     7501 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7561 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7621 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7681 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7741 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7801 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7861 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     7921 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7981 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8041 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8101 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8161 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8221 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8281 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8341 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8401 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8461 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8521 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8581 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8641 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8701 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8761 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8821 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8881 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8941 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9001 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9061 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9121 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9181 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9241 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9301 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9361 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9421 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//
