LOCUS       sequence-166603-        3851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single Integration Vector TRP - Cloning vector for single 
            integration into the TRP1 locus of S.cerevisiae containing two MCS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: SIVt
FEATURES             Location/Qualifiers
     source          1..3851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(70..133)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     411..428
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     412..428
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(457..473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(457..473)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    474..507
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        544..887
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    1813..1846
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     1847..1866
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1847..1865
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1874..1981
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     1898..1914
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1898..1914
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1948..1964)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1949..1965)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(1994..2012)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(2301..2889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2381..2400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3060..3851,1..69))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3683..3702
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
       61 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      121 gagcggatac atatttgaag tatcgttcga attaattaat aatggtagtt ataagaaaga
      181 gaccgagtta gggacagtta gaggcggtgg agatattcct tatggcatgt ctggcgatga
      241 taaaactttt caaacggcag ccccgatcta aaagagctga cagggaaatg gtcagaaaaa
      301 gaaacgtgca cccgcccgtc tggacgcgcc gctcacccgc acggcagaga ccaatcagta
      361 aaaatcaacg tttaacgaca ttactatata tataatatag gaagcattta tgtaaaacga
      421 cggccagtgc atgcgtatcg gacgtctccc ttcatggtca tagctgtttc ctgataactt
      481 cgtataatgt atgctatacg aagttattag cttgcctcgt ccccgccggg tcacccggcg
      541 agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca
      601 tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc
      661 atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgcc tcagacctgc
      721 gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag
      781 agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa
      841 atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccatg agtgtcataa
      901 actttaccgg atcgagtgga cctctcgtca aggtatgcgg gctccagtcc actgaagcag
      961 cggagtgtgc cctagactct gatgccgatc tcttgggcat tatctgcgtc ccgaatagga
     1021 aacgcaccat tgatcctgta atcgcgcgca agatttcttc gctcgtaaag gcctacaaga
     1081 actcttcagg aacgccgaag tatttagtcg gtgtttttcg taatcagcct aaagaggacg
     1141 tattggcact cgtaaacgac tacggcatag atatagtgca gttacatggg gacgagagct
     1201 ggcaggaata tcaagagttc ctgggcttgc ctgtcattaa acgactggtt tttccgaaag
     1261 attgtaatat ccttttgtca gcagcgtcgc agaaaccgca ctcattcata ccgctcttcg
     1321 actcggaagc aggcggtaca ggggaactac tggactggaa tagtataagt gactgggtcg
     1381 gacggcaaga gagtccggag tccttacatt ttatgctggc cgggggttta acccccgaaa
     1441 acgtgggaga tgccctccga ttaaatgggg ttatcggtgt cgatgtatca ggtggggtgg
     1501 agacgaatgg cgtgaaggac tcaaataaaa tcgctaattt tgtgaaaaac gctaagaaat
     1561 gagaatattg ataaatttaa attttggaat gagggttgtc ctttccttta caatagaggc
     1621 atttgagtga gtaggtttta tcaccttctt gctcttatta aatcgcagtt gtctttccgg
     1681 tatgtgcttg actgaggaac aatattttat tcatgataca tttccatgct acgcatatcg
     1741 tcatccacat ggaaggtaat tttcttctgt ggcctttcat atagttgtga atggtagaac
     1801 aatcttcata taataacttc gtataatgta tgctatacga agttattaat acgactcact
     1861 atagggcgaa ttggagctcc accgcggtgg cggccgctct agaactagtg gatcccccgg
     1921 gctgcaggaa ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac
     1981 ccagcttttg ttccctttag tgagggttaa tttcctgagt atttatttaa gtattgtttg
     2041 tgcacttgcc tgcaagcctt ttgaaaagca agcataaatg atctaaacat aaaatctgta
     2101 aaataacaag atgtaaagat aatgctaaat catttggctt tttgattgat tgtacaggaa
     2161 aatatacatc gcaggggttt gacttttacc atttcaccgc aatggaatca aacttgttga
     2221 agagaatgtt cacaggcgca tacgctacaa tgacccgatt cttgctagtt aattaattcg
     2281 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2341 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2401 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2461 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2521 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2581 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2641 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2701 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     2761 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2821 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2881 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2941 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3001 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3061 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3121 gttgcctgac tgcccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3181 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3241 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3301 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3361 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3421 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg aaaaaaagcg
     3481 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3541 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3601 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3661 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3721 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3781 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3841 gtttctgggt g
//
