LOCUS       sequence-166607-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Tagging vector HIS/iRFP - Plasmid for tandem infrared RFP 
            (tdiRFP) labeling of endogenous proteins with a HIS selection 
            marker, originating from pSIVh (ID:81091).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTH-ir
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        80..98
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(127..145)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(146..179)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(203..400)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
       61 cggccttcga attaattaat aatacgactc actatagggg acgtccgaat tggagctcca
      121 attcgcccta tagtgagtcg tattaataac ttcgtatagc atacattata cgaagttatc
      181 ggcgttagta tcgaatcgac agcagtatag cgaccagcat tcacatacga ttgacgcatg
      241 atattacttt ctgcgcactt aacttcgcat ctgggcagat gatgtcgagg cgaaaaaaaa
      301 tataaatcac gctaacattt gattaaaata gaacaactac aatataaaaa aactatacaa
      361 atgacaagtt cttgaaaaca agaatctttt tattgtcagt aatgattaca acactccctt
      421 cgtgcttggg acttcagaac ttccagtaag acttgtagcc gcgcgcatgg caacagccag
      481 agatttaaaa gcgctttcag cacgatgatg gtcattacta ccatataagc aggtaacatg
      541 caaagtaatt ccagcagcta ccgaaaagga atatagtaag tgagggatca tttcacagga
      601 caattcccca accttttcac gctttaatcc caaatcaata acagcatagg gccgtcccga
      661 caaatcaact acgcttcttg aaagagcttc gtcaagtgga caataagcat gtccaaatct
      721 tttaacgccg gcaaagttac ccatagcctg cttgaatgca ataccaagtg caatagcagt
      781 atcttctgct gtgtgatgat catcaatgat taaatcacct cttg
//
